A CHIMERIC TRANSACTIVATOR ALLOWS TETRACYCLINE-RESPONSIVE GENE-EXPRESSION IN WHOLE PLANTS
A CHIMERIC TRANSACTIVATOR ALLOWS TETRACYCLINE-RESPONSIVE GENE-EXPRESSION IN WHOLE PLANTS
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DOI：
10.1046/j.1365-313x.1994.5040559.x
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发表时间：
1994-04-01
期刊：
影响因子：
7.2
通讯作者：
GATZ, C
中科院分区：
文献类型：
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作者：
WEINMANN, P;GOSSEN, M;GATZ, C
嵌合转录激活因子tTA是Tn 10编码的泰特阻遏物和单纯疱疹病毒体蛋白VP 16的激活结构域之间的融合体，在转基因烟草植物中稳定表达。它刺激β-葡萄糖醛酸酶（gus）基因从由7个泰特操纵子和一个TATA盒组成的人工启动子的转录。干扰tTA与操纵基因DNA结合的四环素使gus表达降低几个数量级。这种严格的调节表明，该系统可用于构建编码潜在致死基因产物的转基因植物。此外，tTA的特异性和快速失活允许研究RNA和蛋白质的稳定性。
The chimeric transcriptional activator tTA, a fusion between the Tn10 encoded Tet repressor and the activation domain of the Herpes simplex virion protein VP16, was stably expressed in transgenic tobacco plants. It stimulates transcription of the beta-glucuronidase (gus) gene from an artificial promoter consisting of 7 tet operators and a TATA-box. Tetracycline, which interferes with binding of tTA to operator DNA, reduces gus expression over several orders of magnitude. This stringency of regulation suggests that the system can be used to construct transgenic plants encoding a potentially lethal gene product. Furthermore, the specific and fast inactivation of tTA allows study of the stability of RNAs and proteins.