Rhodopseudomonas palustris Quorum Sensing Molecule pC-HSL Induces Systemic Resistance to TMV Infection via Upregulation of NbSIPK/NbWIPK Expressions in Nicotiana benthamiana
Rhodopseudomonas palustris Quorum Sensing Molecule pC-HSL Induces Systemic Resistance to TMV Infection via Upregulation of NbSIPK/NbWIPK Expressions in Nicotiana benthamiana
复制标题
沼泽红假单胞菌群体感应分子 pC-HSL 通过上调本塞姆氏烟草中 NbSIPK/NbWIPK 的表达来诱导对 TMV 感染的全身抵抗。
DOI：
10.1094/phyto-05-20-0177-r
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发表时间：
2021-03-01
期刊：
影响因子：
3.2
通讯作者：
Su, Pin
中科院分区：
文献类型：
--
作者：
Du, Xiaohua;Huang, Renyan;Su, Pin
G-阴性细菌产生大量的N-酰基高丝氨酸内酯（AHL），可以作为群体感应（QS）信号分子。AHL还已知调节各种植物生物活性。p-Coumaroyl-homoserine lactone（pC-HSL）是光合细菌Rhodopyramidpalustris（R. palustris）。pC-HSL在R.沼泽和植物尚未调查。本文研究了pC-HSL对植物免疫的影响，发现QS分子能诱导本氏烟草（Nicotianabenthamiana）对烟草花叶病毒（Tobacco mosaic virus，TMV）的系统抗性。benthamiana）。结果表明，pC-HSL处理可以延长两个促分裂原相关蛋白激酶（MAPK）基因的激活（即，NbSIPK和NbWIPK）并增强转录因子WRKY 8以及免疫应答标记基因NbPR 1和NbPR 10的表达，导致TMV感染植物中活性氧（ROS）的积累增加。pC-HSL处理能提高POD和SOD两种ROS清除酶活性。敲低N.通过VIGS的pC-HSL诱导的本塞姆氏植物的系统抗性无效或减弱，表明pC-HSL的功能依赖于这两种激酶的活性。同时，pC-HSL预处理的植株在接种TMV后也表现出强烈的NbSIPK和NbWIPK激酶活性诱导。综上所述，我们的研究结果表明，pC-HSL处理增强了植物对TMV感染的抗性，这有助于揭示R.沼泽地及其寄主植物。
G-negative bacteria produce a myriad of N-acyl-homoserine lactones (AHLs) that can function as quorum sensing (QS) signaling molecules. AHLs are also known to regulate various plant biological activities. p-Coumaroyl-homoserine lactone (pC-HSL) is the only QS molecule produced by a photosynthetic bacterium, Rhodopseudomonas palustris (R. palustris). The role of pC-HSL in the interaction between R. palustris and plant has not been investigated. In this study, we investigated the effect of pC-HSL on plant immunity and have found that this QS molecule can induce a systemic resistance to Tobacco mosaic virus (TMV) infection in Nicotiana benthamiana (N. benthamiana). The results show that pC-HSL treatment can prolong the activation of two mitogen-associated protein kinase (MAPK) genes (i.e., NbSIPK and NbWIPK) and enhance the expression of transcription factor WRKY8 as well as immune response marker genes NbPR1 and NbPR10, leading to an increased accumulation of reactive oxygen species (ROS) in the TMV infected plants. Our results also show that pC-HSL treatment can increase activities of two ROS-scavenging enzymes, POD and SOD. Knockdown of NbSIPK or NbWIPK expression in N. benthamiana plants through VIGS nullified or attenuated pC-HSL-induced systemic resistance, indicating that the functioning of pC-HSL relies on the activity of those two kinases. Meanwhile, pC-HSL pre-treated plants also showed a strong induction of kinase activities of NbSIPK and NbWIPK post TMV inoculation. Taken together, our results demonstrate that pC-HSL treatment results in enhanced plant resistance to TMV infection, which is helpful to uncover the outcome of interaction between R. palustris and its host plants.