Identification of atrial natriuretic factor gene transcripts in the central nervous system of the rat.
Identification of atrial natriuretic factor gene transcripts in the central nervous system of the rat.
复制标题
大鼠中枢神经系统心房钠尿因子基因转录本的鉴定。
DOI：
10.1073/pnas.84.8.2175
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
Lewicki,JA
中科院分区：
文献类型：
--
作者：
Gardner,DG;Vlasuk,GP;Baxter,JD;Fiddes,JC;Lewicki,JA
心房利钠因子(ANF)基因转录本已经在大鼠的下丘脑、脑干和大脑皮层中被发现。下丘脑的转录本在总大小(约1100个核苷酸)、5‘端、3’端和核苷酸序列上与在心脏心房合成的转录本相似。由S1核酸酶和引物延伸分析确定的下丘脑转录本的5‘末端位于TATAAAA序列下游约20个核苷酸，该序列被认为决定了转录的开始。在脑干和大脑皮质的RNA中也发现了类似的5‘末端。下丘脑转录产物的3‘末端定位于两个连续的AATAAA序列中每一个序列下游的大约10个核苷酸，这两个序列被认为提供了随后的多腺苷化所需的切割信号。相同的3‘末端存在于心房、心室、肺和垂体的RNA中。为了验证下丘脑基因转录本的同源性，从下丘脑cDNA文库中克隆了编码ANF的部分长度的cDNA克隆，并对其进行了测序。这个下丘脑克隆在其612个核苷酸的每一个位置上都与一个类似的心房cDNA克隆相同。这些发现表明，先前在下丘脑和桥脑被盖特定部位发现的ANF多肽是在这些组织中局部合成的，并支持这种多肽在心血管生理的中枢调节中的作用。
Atrial natriuretic factor (ANF) gene transcripts have been identified in the hypothalamus, brainstem, and cerebral cortex of the rat. The hypothalamic transcripts are similar in overall size (approximately 1100 nucleotides), 5' terminus, 3' terminus, and nucleotide sequence to their counterparts synthesized in the cardiac atria. The 5' terminus of the hypothalamic transcripts, determined by both S1 nuclease and primer-extension analysis, was located approximately 20 nucleotides downstream from the TATAAAA sequence that is thought to dictate the start of transcription. Similar 5' termini were identified in RNA from the brainstem and cerebral cortex. The 3' termini of the hypothalamic transcripts mapped approximately 10 nucleotides downstream from each of two successive AATAAA sequences that are believed to provide the cleavage signal required for subsequent polyadenylylation. The same 3' termini were present in atrial, ventricular, lung, and pituitary RNA. To confirm the identity of the hypothalamic gene transcript, a partial-length cDNA clone encoding ANF was isolated from a hypothalamic cDNA library and sequenced. This hypothalamic clone was identical to an analogous atrial cDNA clone at each of its 612 nucleotide positions. These findings suggest that ANF peptides, previously identified in specific loci of the hypothalamus and pontine tegmentum, are synthesized locally in these tissues and support a role for this peptide in the central regulation of cardiovascular physiology.