Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents.
Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents.
复制标题
使用离子对试剂对肽和蛋白质进行反相高压液相色谱分析。
作者：
W. Hancock;C. A. Bishop;RL Prestidge;Harding;M. Hearn
通过向移动的相中加入亲水性（例如磷酸）或疏水性（例如己磺酸）离子配对试剂或两者，反相高压液相色谱已成功地应用于肽和蛋白质的分析。所描述的实例包括蛋白质如胰岛素、胰高血糖素和1-24 ACTH五乙酸酯（ACTH是促肾上腺皮质激素）。
Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).