Characterization of IgA1 protease as a surface protective antigen of Streptococcus suis serotype 2
Characterization of IgA1 protease as a surface protective antigen of Streptococcus suis serotype 2
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IgA1 蛋白酶作为猪链球菌血清型 2 表面保护抗原的表征
DOI：
10.1016/j.micinf.2015.12.005
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发表时间：
2016-04-01
影响因子：
5.8
通讯作者：
Zhang, Anding
中科院分区：
文献类型：
--
作者：
Fu, Lei;Zhao, Jianqing;Zhang, Anding
猪链球菌2型(SS2)的IgA1蛋白水解酶已被证实与其毒力和免疫原性有关，但其保护作用仍有待评价。本研究发现，用纯化的重组IgA1蛋白酶(600-1926aa)免疫小鼠，可诱导高滴度的抗体滴度，并对致死剂量的SS2攻击具有完全保护作用。此外，我们的研究结果证实，IgA1蛋白水解酶存在于SS2的表面。因此，本研究确定毒力相关蛋白IgA1蛋白为SS2新的表面保护性抗原。(C)2016年巴斯德研究所。爱思唯尔·马森公司出版。版权所有。
IgA1 protease of Streptococcus suis serotype 2 (SS2) has been proven to be relative with virulence and immunogenicity, however, its protective efficacy remained to be evaluated. The present study found evidence that immunization with purified recombinant IgA1 protease (600-1926aa) could induce high IgG antibody titers and could confer complete protection against a challenge with a lethal dose of SS2 in a mouse model. In addition, our findings confirmed that the IgA1 protease distributes on the surface of SS2. Therefore, the present study identified the virulence-associated protein, IgA1 protease, as a novel surface protective antigen of SS2. (C) 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.