Integrase of Mason-Pfizer monkey virus.
Integrase of Mason-Pfizer monkey virus.
复制标题
梅森-辉瑞猴病毒的整合酶。
DOI：
10.1111/j.1432-1033.2004.04386.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Pichova,Iva
中科院分区：
文献类型：
--
作者：
Snasel,Jan;Krejcik,Zdenek;Jencova,Vera;Rosenberg,Ivan;Ruml,Tomas;Alexandratos,Jerry;Gustchina,Alla;Pichova,Iva
The gene encoding an integrase of Mason–Pfizer monkey virus (M‐PMV) is located at the 3′‐end of the pol open reading frame. The M‐PMV integrase has not been previously isolated and characterized. We have now cloned, expressed, isolated, and characterized M‐PMV integrase and compared its activities and primary structure with those of HIV‐1 and other retroviral integrases. M‐PMV integrase prefers untranslated 3′‐region‐derived long‐terminal repeat sequences in both the 3′‐processing and the strand transfer activity assays. While the 3′‐processing reaction catalyzed by M‐PMV integrase was significantly increased in the presence of Mn2+and Co2+and was readily detectable in the presence of Mg2+and Ni2+cations, the strand transfer activity was strictly dependent only on Mn2+. M‐PMV integrase displays more relaxed substrate specificity than HIV‐1 integrase, catalyzing the cleavage and the strand transfer of M‐PMV and HIV‐1 long‐terminal repeat‐derived substrates with similar efficiency. The structure‐based sequence alignment of M‐PMV, HIV‐1, SIV, and ASV integrases predicted critical amino acids and motifs of M‐PMV integrase for metal binding, interaction with nucleic acids, dimerization, protein structure maintenance and function, as well as for binding of human immunodeficiency virus type 1 and Rous avian sarcoma virus integrase inhibitors 5‐CI‐TEP, DHPTPB and Y‐3.
