Long-term monitoring of depolarization-induced exocytosis from adrenal medullary chromaffin cells and pancreatic islet B cells using "perforated patch recording".
Long-term monitoring of depolarization-induced exocytosis from adrenal medullary chromaffin cells and pancreatic islet B cells using "perforated patch recording".
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使用“穿孔贴片记录”长期监测肾上腺髓质嗜铬细胞和胰岛 B 细胞的去极化诱导的胞吐作用。
DOI：
10.1111/j.1749-6632.1991.tb36528.x
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发表时间：
1991
影响因子：
5.2
通讯作者：
Misler,S
中科院分区：
文献类型：
--
作者：
Gillis,KD;Pun,RY;Misler,S
(1)穿孔贴片记录的膜电容测量提供了以前所未有的时间分辨率和稳定性研究单个内分泌细胞的去极化-分泌耦合(DSC)过程的可能性。(2)分泌儿茶酚胺的肾上腺嗜铬细胞和分泌胰岛素的胰腺B细胞的早期结果支持了长期以来的观点，即DSC背后的钙依赖过程基本上类似于神经末梢。(3)未来使用这些方法的实验将被证明有助于区分那些对兴奋性具有主要影响的体液物质的影响，以及那些影响分泌过程本身的钙离子进入。
(1) Membrane capacitance measurements using perforated patch recording offer the possibility of studying the process of depolarization-secretion coupling (DSC) in single endocrine cells with unprecedented time resolution and stability.(2) Early results with catecholamine-secreting adrenal chromaffin cells and insulin-secreting pancreatic B cells support longstanding ideas that the Ca (2+)-dependent processes underlying DSC are fundamentally similar to those of nerve terminals.(3) Future experiments using these approaches should prove useful in sorting out those effects of humoral substances that have a predominant effect on excitability and Ca2+ entry from those that affect the secretory process itself.