Probability of detecting Porcine reproductive and respiratory syndrome virus infection using pen-based swine oral fluid specimens as a function of within-pen prevalence
Probability of detecting Porcine reproductive and respiratory syndrome virus infection using pen-based swine oral fluid specimens as a function of within-pen prevalence
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DOI：
10.1177/1040638713481471
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发表时间：
2013-05-01
影响因子：
1.5
通讯作者：
Zimmerman, Jeffrey
中科院分区：
文献类型：
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作者：
Olsen, Chris;Wang, Chong;Zimmerman, Jeffrey
基于笔的口腔液取样已被证明是猪群传染病监测的一种有效方法。为了更好地解释诊断结果，必须建立基于笔的口腔液样本的口腔液测定（基于抗体和核酸）的性能。因此，本研究的目的是确定在已知PRRSV流行的猪圈的口腔液样本中检测到猪繁殖与呼吸综合征病毒（PRRSV）感染的概率。在1个商业猪舍中，通过在每个猪圈中放置固定数量（0、1、3、5或9头）的PRRSV阳性猪（接种PRRSV修饰活病毒后14天），将25个猪圈分配到5个PRRSV流行水平（0%、4%、12%、20%或36%）中的1个水平。在放置接种猪之前，从每个猪圈收集1份口服液样本。随后，每个笔采集5份口服液样本，共计150份样本。为了确认个体猪的PRRSV状态，对535头PRRSV阴性猪和90头PRRSV疫苗接种猪的血清样本进行了PRRSV抗体和PRRSV RNA检测。在6个实验室对150份笔制口腔液样本进行PRRSV抗体和PRRSV RNA检测。在6个实验室检测的100个猪圈样本中，有>= 1阳性猪（>= 4%），PRRSV RNA的平均阳性率为62%，PRRSV抗体的平均阳性率为61%。这些结果支持在商品猪种群中使用基于笔的口腔液取样进行PRRSV监测。
Pen-based oral fluid sampling has proven to be an efficient method for surveillance of infectious diseases in swine populations. To better interpret diagnostic results, the performance of oral fluid assays (antibody- and nucleic acid-based) must be established for pen-based oral fluid samples. Therefore, the objective of the current study was to determine the probability of detecting Porcine reproductive and respiratory syndrome virus (PRRSV) infection in pen-based oral fluid samples from pens of known PRRSV prevalence. In 1 commercial swine barn, 25 pens were assigned to 1 of 5 levels of PRRSV prevalence (0%, 4%, 12%, 20%, or 36%) by placing a fixed number (0, 1, 3, 5, or 9) of PRRSV-positive pigs (14 days post PRRSV modified live virus vaccination) in each pen. Prior to placement of the vaccinated pigs, 1 oral fluid sample was collected from each pen. Thereafter, 5 oral fluid samples were collected from each pen, for a total of 150 samples. To confirm individual pig PRRSV status, serum samples from the PRRSV-negative pigs (n = 535) and the PRRSV vaccinated pigs (n = 90) were tested for PRRSV antibodies and PRRSV RNA. The 150 pen-based oral fluid samples were assayed for PRRSV antibody and PRRSV RNA at 6 laboratories. Among the 100 samples from pens containing >= 1 positive pig (>= 4% prevalence) and tested at the 6 laboratories, the mean positivity was 62% for PRRSV RNA and 61% for PRRSV antibody. These results support the use of pen-based oral fluid sampling for PRRSV surveillance in commercial pig populations.