The improved organ maintenance of the human sebaceous gland: Modeling in vitro the effects of epidermal growth factor, androgens, estrogens, 13-cis retinoic acid, and phenol red
The improved organ maintenance of the human sebaceous gland: Modeling in vitro the effects of epidermal growth factor, androgens, estrogens, 13-cis retinoic acid, and phenol red
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DOI：
10.1111/1523-1747.ep12343608
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发表时间：
1996-03-01
影响因子：
6.5
通讯作者：
Kealey, T
中科院分区：
文献类型：
--
作者：
Guy, R;Ridden, C;Kealey, T
我们以前曾报道，人皮脂腺可以作为完整的器官维持长达14天，完全保留了新细胞形成的生理速率和模式，但我们也报道了新形成的细胞不能正常分化，导致体外脂肪生成的进行性丧失，我们现在表明，这种异常的皮脂腺细胞分化是由于存在表皮生长因子（EGF）和酚红在我们的维持培养基。在他们的情况下，人类皮脂腺显然保留在体内的细胞分裂和脂肪生成率超过7天的维护除了保留原位形态。这在再加入10 ng EGF/ml和10 mg酚红/ml时是可逆的。添加600 pM的17 β-雌二醇导致维持7天以上的脂肪生成速率显著下降，而不影响细胞分裂速率。这种作用显然是由于新形成的皮脂细胞的异常分化。1 nM睾酮和1 nM双氢睾酮（DHT）对7 d内脂肪生成的细胞分裂速率均无任何影响。然而，在酚红存在下，1 nM睾酮或1 nM DHT导致7 d内维持的脂肪生成速率显著降低。一微摩尔13-顺式维甲酸引起的脂肪生成率显着降低超过7天，在存在和不存在的酚红，这些研究结果表明，我们可以模拟类固醇，EGF，和13-顺式维甲酸在体外的生理效应。
We have previously reported that human sebaceous glands can be maintained for up to 14 d as whole organs with full retention of the physiological rate and pattern of new cell formation, but we have also reported that the newly formed cells did not differentiate normally, causing a progressive loss of lipogenesis in vitro, We now show that this abnormal sebocyte differentiation was attributable to the presence of epidermal growth factor (EGF) and phenol red in our maintenance medium. In their absence, human sebaceous glands apparently retain in vivo rates of cell division and lipogenesis over 7 d of maintenance in addition to a retention of in situ morphology. This is reversible on the re-addition of 10 ng EGF/ml and 10 mg phenol red/ml. The addition of 600 pM 17 beta-estradiol results in a significant fall in rate of lipogenesis over 7 d of maintenance, without affecting the rate of cell division, This effect is apparently due to abnormal differentiation of newly formed sebocytes. Neither 1 nM testosterone nor 1 nM dihydrotestosterone (DHT) has any effect on rates of cell division of lipogenesis over 7 d, In the presence of phenol red, however, 1 nM testosterone or 1 nM DHT cause a significant reduction in the rate of lipogenesis over 7 d of maintenance. One micromolar 13-cis retinoic acid caused a significant reduction in the rate of Lipogenesis over 7 d in both the presence and absence of phenol red, These findings show that we can model the physiological effects of steroids, EGF, and 13-cis retinoic acid in vitro.