Sarpeptins A and B, Lipopeptides Produced by Streptomyces sp. KO-7888 Overexpressing a Specific SARP Regulator
Sarpeptins A and B, Lipopeptides Produced by Streptomyces sp. KO-7888 Overexpressing a Specific SARP Regulator
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DOI：
10.1021/acs.jnatprod.9b00074
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发表时间：
2019-08-01
影响因子：
5.1
通讯作者：
Nakashima, Takuji
中科院分区：
文献类型：
--
作者：
Koomsiri, Wilaiwan;Inahashi, Yuki;Nakashima, Takuji
链霉菌KO-7888的全基因组分析揭示了与沉默生物合成基因簇相关的多种途径特异性转录调控基因。链霉菌抗生素调节蛋白基因，speR，位于一个新的非核糖体肽合成酶（NRPS）基因簇附近，在野生型菌株中过表达。得到的链霉菌KO-7888重组菌株产生两种新的脂肽，沙肽素A和B。通过高分辨电喷雾质谱、核磁共振分析和先进的Marveland方法鉴定了它们的结构。不同的模块部分相应的NRPS生物合成基因簇的特点，并提出了生产脂肽链的装配线。
Whole genome analysis of Streptomyces sp. KO-7888 has revealed various pathway-specific transcriptional regulatory genes associated with silent biosynthetic gene clusters. A Streptomyces antibiotic regulatory protein gene, speR, located adjacent to a novel nonribosomal peptide synthetase (NRPS) gene cluster, was overexpressed in the wild-type strain. The resulting recombinant strain of Streptomyces sp. KO-7888 produced two new lipopeptides, sarpeptins A and B. Their structures were elucidated by high-resolution electrospray ionization mass spectrometry, NMR analysis, and the advanced Marfey's method. The distinct modular sections of the corresponding NRPS biosynthetic gene cluster were characterized, and the assembly line for production of the lipopeptide chain was proposed.