Discovery of transcriptional regulators and signaling pathways in the developing pituitary gland by bioinformatic and genomic approaches.
Discovery of transcriptional regulators and signaling pathways in the developing pituitary gland by bioinformatic and genomic approaches.
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DOI：
10.1016/j.ygeno.2008.11.010
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发表时间：
2009-05
期刊：
影响因子：
4.4
通讯作者：
Camper, Sally A.
中科院分区：
文献类型：
--
作者：
Brinkmeier, Michelle L.;Davis, Shannon W.;Carninci, Piero;MacDonald, James W.;Kawai, Jun;Ghosh, Debashis;Hayashizaki, Yoshihide;Lyons, Robert H.;Camper, Sally A.
我们报道了一个小鼠胚胎垂体转录组的目录，包括5个cDNA文库，包括E12.5和E14.5的野生型组织，E14.5的Prop 1df/df突变体，以及两个cDNA消减：E14.5 WT-E14.5 Prop 1df/df和E14. 5 WT-E12.5 WT。DNA序列信息被组装到一个可搜索的数据库中，其中基因本体论术语代表12，009个表达基因。我们通过检测大多数已知的同源框基因转录因子cDNA来验证文库的覆盖率。总共检测到45个同源盒基因作为垂体转录组的一部分，代表了最预期的基因，这验证了库覆盖率，以及许多新基因，强调了该资源作为发现工具的实用性。我们对具有新垂体表达的信号通路成员采取了类似的方法，发现了157个与BMP、FGF、WNT、SHH和NOTCH通路相关的基因。这些基因是垂体发育和功能调节因子的激动人心的候选者。
We report a catalog of the mouse embryonic pituitary gland transcriptome consisting of five cDNA libraries including wild type tissue from E12.5 and E14.5, Prop1df/df mutant at E14.5, and two cDNA subtractions: E14.5 WT-E14.5 Prop1df/df and E14.5 WT-E12.5 WT. DNA sequence information is assembled into a searchable database with gene ontology terms representing 12,009 expressed genes. We validated coverage of the libraries by detecting most known homeobox gene transcription factor cDNAs. A total of 45 homeobox genes were detected as part of the pituitary transcriptome, representing most expected ones, which validated library coverage, and many novel ones, underscoring the utility of this resource as a discovery tool. We took a similar approach for signaling-pathway members with novel pituitary expression and found 157 genes related to the BMP, FGF, WNT, SHH and NOTCH pathways. These genes are exciting candidates for regulators of pituitary development and function.
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影响因子：
2.5
作者：
Douglas, KR;Brinkmeier, ML;Camper, SA
通讯作者：
Camper, SA
影响因子：
2.7
作者：
Ermakova, Galina V.;Solovieva, Elena A.;Zaraisky, Andrey G.
通讯作者：
Zaraisky, Andrey G.
影响因子：
--
作者：
Bailey, JS;Rave-Harel, N;Mellon, PL
通讯作者：
Mellon, PL
影响因子：
3.7
作者：
Bentley, CA;Zidehsarai, MP;Roberts, VJ
通讯作者：
Roberts, VJ
影响因子：
--
作者：
Charles, MA;Suh, H;Gage, PJ
通讯作者：
Gage, PJ