Bimodal patterns of floral gene expression over the two seasons that kiwifruit flowers develop.
Bimodal patterns of floral gene expression over the two seasons that kiwifruit flowers develop.
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DOI：
10.1034/j.1399-3054.2001.1110318.x
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发表时间：
2001-03
影响因子：
6.4
通讯作者：
E. F. Walton;E. Podivinsky;R. Wu
中科院分区：
文献类型：
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作者：
E. F. Walton;E. Podivinsky;R. Wu
从猕猴桃（Actinidia deliciosa [A. Chev.] C. F. Liang和A. R. Ferguson），分别命名为ALF和AAP 1。北方杂交分析表明，ALF和AAP 1在发育中的一级腋芽及其随后的芽中具有双峰的年度表达模式。这种表达模式与猕猴桃中观察到的腋芽、花和果实发育的2年周期一致。表达的第一个时期是早期的一阶芽发育（春末的第一个生长季节），当二级分生组织开始，和第二个，大约10个月后，当这些分生组织分化花（春末的第二个生长季节）。在第一个生长季节的晚春期间收集的腋芽原位杂交分析表明，ALF的表达在整个发展中的一阶芽和AAP 1的表达被定位在发展中的二阶腋芽分生组织。在第二个生长季节的春季，ALF和AAP 1的转录物积累定位于分化的花。我们的研究结果表明，重要的发育事件发生在猕猴桃一阶腋芽发育（春季的第一个生长季节）非常早，这很可能包括花的承诺（唤起）。
Polymerase chain reaction fragments with homology to the Arabidopsis floral meristem identity genes LEAFY and APETALA1 have been isolated from kiwifruit (Actinidia deliciosa [A. Chev.] C. F. Liang and A. R. Ferguson) and have been named ALF and AAP1, respectively. Northern hybridisation analyses have shown that ALF and AAP1 have bimodal patterns of annual expression in developing first-order axillary buds and their subsequent shoots. This pattern of expression is consistent with the 2-year cycle of axillary bud, flower and fruit development observed in kiwifruit. The first period of expression was early in first-order bud development (late spring of the first growing season), when second-order meristems are initiated, and the second, approximately 10 months later, when those meristems differentiate flowers (late spring of the second growing season). In situ hybridisation analyses on axillary buds collected during late spring of the first growing season have shown ALF expression throughout the developing first-order buds and AAP1 expression was localised in developing second-order axillary meristems. During the spring of the second growing season, transcript accumulation for both ALF and AAP1 is localised in differentiating flowers. Our results show that important developmental events are occurring very early in kiwifruit first-order axillary bud development (spring of the first growing season) and it is likely that this includes floral commitment (evocation).