ISOLATION AND AMINO ACID SEQUENCE OF ELEDOISIN, ACTIVE ENDECAPEPTIDE OF POSTERIOR SALIVARY GLANDS OF ELEDONE
ISOLATION AND AMINO ACID SEQUENCE OF ELEDOISIN, ACTIVE ENDECAPEPTIDE OF POSTERIOR SALIVARY GLANDS OF ELEDONE
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DOI：
10.1016/0003-9861(63)90533-2
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发表时间：
1963-01-01
影响因子：
3.9
通讯作者：
ERSPAMER, V
中科院分区：
文献类型：
--
作者：
ANASTASI, A;ERSPAMER, V
将地中海八臂头足类动物 Eledone 后唾液腺的粗甲醇提取物依次进行碱性氧化铝色谱（或血清白蛋白吸附）、离子交换色谱和逆流分配。由此获得了这些提取物的活性成分，即多肽草红素，以纯净的形式获得。全部和部分酸水解、胰蛋白酶和胰凝乳蛋白酶裂变以及端基测定实验表明，香芹素是一种内十肽，其氨基酸组成和序列如下：Pyr-Pro-Ser-Lys-Asp(OH)-Ala-Phe-lle-Gly-Leu-Met-NH2。
Crude methanol extracts of posterior salivary glands of Eledone, a Mediterranean eight-armed cephalopod, were subjected successively to chromatography on alkaline alumina (or to absorption on serum albumin), to ion-exchange chromatography, and to countercurrent distribution. The active constituent of these extracts, the polypeptide eledoisin, was thus obtained in a pure form. Total and partial acid hydrolysis, fission with trypsin and chymotrypsin, and end-group determination experiments demonstrated that eledoisin is an endecapeptide having the following amino acid composition and sequence Pyr-Pro-Ser-Lys-Asp(OH)-Ala-Phe-lle-Gly-Leu-Met-NH2.