Detection of RTX toxin gene in Vibrio cholerae by PCR
Detection of RTX toxin gene in Vibrio cholerae by PCR
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DOI：
10.1128/jcm.39.7.2594-2597.2001
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发表时间：
2001-07-01
影响因子：
9.4
通讯作者：
Yam, WC
中科院分区：
文献类型：
--
作者：
Chow, KH;Ng, TK;Yam, WC
最近发现的霍乱弧菌毒素基因RTX （repeat in toxin，重复毒素）基因的聚合酶链反应（PCR）被开发出来。在166株临床和环境分离株中；通过聚合酶链反应和HEp-2细胞毒性试验发现，在世界各地引起流行的霍乱和散发性霍乱病例均具有产毒性。在基因簇中含有缺失的经典生物型标准菌株在两种检测中均表现出阴性结果。这是第一个用于区分O1型霍乱弧菌经典生物型菌株与El - Tor型菌株以及其他非O1血清群（包括O139血清群）菌株的快速基因分型方法。该PCR方法还专门检测霍乱弧菌中RTX毒素基因，作为副溶血性弧菌、腹泻性大肠杆菌、气单胞菌等临床分离株，RTX毒素特异性PCR和HEp-2细胞毒性试验均为阴性。这些发现突出了霍乱弧菌RTX毒素的特点，它们在细菌致病性中的作用有待进一步研究。
A PCR that amplifies a recently discovered Vibrio cholerae RTX (repeat in toxin) toxin gene was developed. Among 166 clinical and environmental isolates of V; cholerae causing epidemics and sporadic cases of cholera in various parts of the world, all were found to be toxigenic by both PCR and HEp-2 cell cytotoxicity assay. Standard strains of the classical biotype containing a deletion within the gene cluster exhibited negative results by both assays. This is the first rapid genotyping method for differentiation of V, cholerae O1 classical biotype strains from El Tor biotype strains as well as strains of other non-O1 serogroups including serogroup O139, The PCR assay that was developed also specifically detects RTX toxin genes in V. cholerae, as clinical isolates of Vibrio parahaemolyticus, diarrheagenic Escherichia coli, Aeromonas species, and Plesiomonas species were all negative by the RTX toxin-specific PCR as well as the HEp-2 cytotoxicity assay. These findings highlight the characteristics of the RTX toxins in V. cholerae, Their role in the pathogenicity of the bacterium requires further investigation.