Ciliary band gene expression patterns in the embryo and trochophore larva of an indirectly developing polychaete
Ciliary band gene expression patterns in the embryo and trochophore larva of an indirectly developing polychaete
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DOI：
10.1016/j.modgep.2007.01.007
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发表时间：
2007-04-01
影响因子：
1.2
通讯作者：
Arandi-Forosani, Navid
中科院分区：
文献类型：
--
作者：
Arenas-Mena, Cesar;Wong, Kimberly Suk-Ying;Arandi-Forosani, Navid
间接发育的旋毛虫担轮幼虫有纤毛带，具有运动和进食功能。口前原轮纤毛带是环节动物和软体动物胚胎的第一个分化器官。在这里，我们报告的几个纤毛带标记的表达在胚胎发育和早期幼虫阶段的间接发展多毛类Hydroides elegans。与小窝蛋白、β-微管蛋白、α-微管蛋白和tektin相似的基因在8个主要原轮前体1 q（221）和1 q（212）中表达。卵裂球1 q（221）和1 q（212）在其1 q（22）和1 q（21）前体的互补不对称分裂后位于同一赤道纬度。此外，小窝蛋白和α-微管蛋白在后牙弓和口缘睫状带中表达。小窝蛋白在前肠纤毛细胞中表达，α-微管蛋白在顶簇纤毛细胞中表达。β-胸腺素同源物的表达仅限于1 q（122）和1 q（121）卵裂球，其位于8个原轮细胞1 q（221）和1 q（212）的正上方并与其密切相关。此外，fl-胸腺素同系物在后肠和顶区中具有瞬时表达。所有这些基因的表达为睫状带和其他纤毛器官的早期特化提供了一个里程碑。(c)2007 Elsevier B. V.保留所有权利。
The trochophore larvae of indirectly developing spiralians have ciliary bands with motor and feeding functions. The preoral prototroch ciliary band is the first differentiating organ in annelid and mollusk embryos. Here we report the expression of several ciliary band markers during embryogenesis and early larval stages of the indirectly developing polychaete Hydroides elegans. Genes with similarity to caveolin, beta-tubulin, a-tubulin, and tektin are expressed in the eight primary prototroch precursors, 1q(221) and 1q(212). Blastomeres 1q(221) and 1q(212) locate at the same equatorial latitude after the complementary asymmetric division of their 1q(22) and 1q(21) precursors. In addition, caveolin and a-tubulin are expressed in the metatroch and adoral ciliary zone. Caveolin is expressed in foregut ciliated cells, and a-tubulin is expressed in apical tuft ciliated cells. The expression of a beta-thymosin homolog is restricted to 1q(122) and 1q(121) blastomeres, which locate just above and in close association with the eight primary prototroch cells 1q(221) and 1q(212). In addition, the fl-thymosin homolog has a transient expression in the hindgut and apical zone. The expression of all these genes provides a landmark for the early specification of ciliary bands and other ciliated organs. (c) 2007 Elsevier B.V. All rights reserved.