In vivo assessment of acetylcholine-releasing function at cardiac vagal nerve terminals.
In vivo assessment of acetylcholine-releasing function at cardiac vagal nerve terminals.
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心脏迷走神经末梢乙酰胆碱释放功能的体内评估。
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Kenji Sunagawa
中科院分区：
文献类型：
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作者：
T. Kawada;T. Yamazaki;T. Akiyama;T. Shishido;M. Inagaki;K. Uemura;T. Miyamoto;M. Sugimachi;Hiroshi Takaki;Kenji Sunagawa
我们研究了心脏微透析测定的ACh浓度是否提供了麻醉猫在各种迷走神经刺激和调节条件下左心室ACh水平的信息。局部给予KCl（n = 5）和哇巴因（n = 7）使透析液中的ACh浓度从基线值0.6 +/- 0.5 nmol/l分别显著增加至4.3 +/- 0.8和7.3 +/- 1.3 nmol/l。静脉注射苯基双胍（n = 5）和苯乙双胍（n = 6）可显著增加ACh浓度，分别为5.4 +/- 0.9和6.0 +/- 1.5 nmol/l，表明Bezold-Jorich反射和动脉压力感受器反射影响心肌ACh水平。通过局部给予河豚毒素（n = 6）、半胆碱-3（n = 6）和vesamicol（n = 5）来调节迷走神经末梢功能，可显著抑制电刺激诱导的ACh释放，分别从20.4 +/- 3.9降至0.6 +/- 0.1、7.2 +/- 1.9和2.7 +/- 0.6 nmol/l。心率从120次/分增加到200次/分显著降低了电迷走神经刺激期间的心肌ACh水平，表明ACh的心率依赖性洗脱。我们的结论是，心脏微透析测定的ACh浓度提供了信息，在体内各种病理生理条件下的ACh释放和处置。
We examined whether the ACh concentration measured by cardiac microdialysis provided information on left ventricular ACh levels under a variety of vagal stimulatory and modulatory conditions in anesthetized cats. Local administration of KCl (n = 5) and ouabain (n = 7) significantly increased the ACh concentration in the dialysate to 4.3 +/- 0.8 and 7.3 +/- 1.3 nmol/l, respectively, from the baseline value of 0.6 +/- 0.5 nmol/l. Intravenous administration of phenylbiguanide (n = 5) and phenylephrine (n = 6) significantly increased the ACh concentration to 5.4 +/- 0.9 and 6.0 +/- 1.5 nmol/l, respectively, suggesting that the Bezold-Jarisch and arterial baroreceptor reflexes affected myocardial ACh levels. Modulation of vagal nerve terminal function by local administration of tetrodotoxin (n = 6), hemicholinium-3 (n = 6), and vesamicol (n = 5) significantly suppressed the electrical stimulation-induced ACh release from 20.4 +/- 3.9 to 0.6 +/- 0.1, 7.2 +/- 1.9, and 2.7 +/- 0.6 nmol/l, respectively. Increasing the heart rate from 120 to 200 beats/min significantly reduced the myocardial ACh levels during electrical vagal stimulation, suggesting a heart rate-dependent washout of ACh. We conclude that ACh concentration measured by cardiac microdialysis provides information regarding ACh release and disposition under a variety of pathophysiological conditions in vivo.
大鼠 Bezold-Jarisch 反射的髓质通路。
DOI：
10.1152/ajpregu.1992.263.6.r1195
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
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作者：
Verberne,AJ;Guyenet,PG
通讯作者：
Guyenet,PG
犬心室内在神经支配的来源：功能研究。
DOI：
10.1152/ajpheart.1987.252.3.h638
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
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作者：
Blomquist,TM;Priola,DV;Romero,AM
通讯作者：
Romero,AM