Aggressive behavior in adult male mice elevates serum nerve growth factor levels.
Aggressive behavior in adult male mice elevates serum nerve growth factor levels.
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DOI：
10.1152/ajpendo.1986.250.4.e386
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发表时间：
1986-04
期刊：
影响因子：
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通讯作者：
J. Lakshmanan
中科院分区：
文献类型：
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作者：
J. Lakshmanan
通过使用特异性和敏感性β-神经生长因子放射免疫测定法（β NGF-RIA），在各种生理条件下测定成年雄性Swiss-Webster小鼠的血清β NGF浓度。在动物供应商到达当天处死的动物显示血清β NGF水平的广泛变化（3-15 ng β NGF/ml）。如果动物在死亡前在单独饲养条件下饲养至少6天，则这些水平显著降低。先前单独圈养的动物之间的社会接触引起的攻击性血清β-NGF水平相对于非攻击性小鼠的血清β-NGF浓度提高了两个数量级。死亡前5天给予丙酸替丁酯（10 mg/动物）治疗未升高单独饲养动物的血清β NGF水平。然而，激素预处理显着增加血清β-神经生长因子水平在侵略性小鼠相比，在激素治疗的非侵略性动物的水平。在死亡前10天去除下颌下舌下唾液腺完全消除了由于侵略引起的血清β-NGF的增加。通过竞争性RIA在攻击性小鼠中测量的高血清β NGF水平通过神经突生长PC-12生物测定系统证实。总之，成年雄性小鼠的血清β-NGF受到环境应激的影响。
By use of a specific and sensitive beta-nerve growth factor radioimmunoassay (beta NGF-RIA), serum beta NGF concentrations were measured in adult male Swiss-Webster mice under various physiological conditions. Animals killed on the day of arrival from the animal supplier showed wide variations (3-15 ng beta NGF/ml) in serum beta NGF levels. These levels significantly decreased if the animals were maintained under individually housed conditions for at least 6 days prior to death. Aggression caused by social contact between previously individually housed animals raised serum beta NGF levels by two orders of magnitude relative to serum beta NGF concentrations in nonaggressive mice. Testosterone propionate treatment (10 mg/animal) 5 days prior to death did not elevate serum beta NGF levels in individually maintained animals. However, the hormone pretreatment markedly augmented serum beta NGF levels in aggressive mice compared with the levels in hormone-treated nonaggressive animals. Removal of the submandibular-sublingual salivary glands 10 days prior to death completely abolished the increase in serum beta NGF due to aggression. The high serum beta NGF levels, as measured by competitive RIA in aggressive mice, were confirmed by a neurite outgrowth PC-12 bioassay system. In conclusion, serum beta NGF in adult male mice is subject to alteration by environmental stress.