Truncated Streptococcus pneumoniae PspA molecules elicit cross-protective immunity against pneumococcal challenge in mice.
Truncated Streptococcus pneumoniae PspA molecules elicit cross-protective immunity against pneumococcal challenge in mice.
复制标题
DOI：
10.1093/infdis/173.2.380
复制
发表时间：
1996-02
期刊：
影响因子：
--
通讯作者：
Rebecca Tart;L. McDaniel;Beth A. Ralph;D. Briles
中科院分区：
文献类型：
--
作者：
Rebecca Tart;L. McDaniel;Beth A. Ralph;D. Briles
肺炎链球菌RX1株的肺炎球菌表面蛋白A(PSPA)免疫小鼠可交叉保护小鼠免受不同肺炎球菌的攻击。截短的RX1 PSPA由192-588个氨基酸组成，对小鼠强毒株WU2具有保护作用。研究了其他PspA的同源区域也可能引起交叉保护的可能性。根据RX1 PSPA基因序列设计寡核苷酸引物，从15株不同的肺炎球菌中扩增出染色体DNA。用7株代表囊膜类型3、4、5、6A和6B的菌株评估了三种重组PspA对小鼠攻击的保护能力。三个截短的PspA中的两个分别对所检测的71%-100%的肺炎链球菌激发株产生交叉保护作用。这些数据表明，这项技术可能有助于产生不同的PspA，以包括在广泛保护的肺炎球菌疫苗中。
Immunization with pneumococcal surface protein A (PspA) from Streptococcus pneumoniae strain Rx1 cross-protects mice against challenge with diverse pneumococci. Truncated Rx1 PspA, consisting of amino acids 192-588, elicits protection against the mouse-virulent strain WU2. The possibility that homologous regions of other PspAs could also elicit cross-protection was investigated. Oligonucleotide primers designed according to the Rx1 pspA gene sequence were used to amplify chromosomal DNA from 15 diverse pneumococci. Three recombinant PspAs were evaluated for their ability to elicit protection in mice against challenge with 7 strains representing capsular types 3, 4, 5, 6A, and 6B. Two of the three truncated PspAs each elicited cross-protection against 71%-100% of the S. pneumoniae challenge strains examined. These data suggest that this technique may be useful for the generation of diverse PspAs for inclusion in a broadly protective pneumococcal vaccine.