A minimally invasive, low-stress method for serial blood collection in aging mice
A minimally invasive, low-stress method for serial blood collection in aging mice
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DOI：
10.1080/20010001.2019.1647400
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发表时间：
2019-07-26
期刊：
影响因子：
--
通讯作者：
Zhu, Lida
中科院分区：
文献类型：
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作者：
Bjorner, Marianne;Zhu, Lida
血液学分析是小鼠衰老研究中实时健康监测和免疫分析的有效和有价值的工具。然而，许多常用的小鼠血液采样技术与随着年龄增长的连续监测不相容，因为它们可能涉及麻醉，导致严重的压力，或需要大量的血液。本技术报告描述了一种方便的相对非侵入性程序，通过优化的尾血采集方法，然后进行瑞氏-姬姆萨和特克染色，对C57 Bl/6小鼠的白色血细胞进行计数。这种技术可以在不到1分钟的时间内对未麻醉的小鼠进行，只需使用几微升的血液，压力最小。白色血细胞分析可以包括但不限于总的和分类的白色血细胞计数和细胞形态。收集的低血量对于需要定期和连续监测的长期实验中的衰老小鼠是理想的。
Hematologic analysis is an efficient and valuable tool for real-time health monitoring and immune analysis in mouse aging studies. However, many frequently used blood sampling techniques in mice are incompatible with continuous monitoring with increasing age, as they may involve anesthesia, cause severe stress, or require a high volume of blood. This technical report describes a convenient relatively noninvasive procedure for counting white blood cells in C57Bl/6 mice by an optimized tail blood collection method followed by Wright-Giemsa and Turk staining. This technique can be performed on unanesthetized mice in less than 1 min with minimal stress using only a few microliters of blood. White blood cell analysis can include but is not limited to total and differential white blood cell count and cell morphology. The low blood volume collected is ideal for aging mice in longer-term experiments requiring regular and continuous monitoring.