MicroRNA profiling of the murine hematopoietic system.
MicroRNA profiling of the murine hematopoietic system.
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DOI：
10.1186/gb-2005-6-8-r71
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发表时间：
2005
期刊：
影响因子：
12.3
通讯作者：
Kosik, Kenneth S
中科院分区：
文献类型：
--
作者：
Monticelli, Silvia;Ansel, K Mark;Xiao, Changchun;Socci, Nicholas D;Krichevsky, Anna M;Thai, To-Ha;Rajewsky, Nikolaus;Marks, Debora S;Sander, Chris;Rajewsky, Klaus;Rao, Anjana;Kosik, Kenneth S
第一份关于造血系统细胞中系统的miRNA图谱的报告表明，除了调节对特定细胞谱系的承诺外，miRNAs可能在细胞分化和细胞鉴定中具有一般作用。MicroRNAs(MiRNAs)是最近发现的一类非编码RNA基因，它在转录后调节基因的表达。越来越清楚的是，miRNAs在发育过程中对基因表达的调控发挥着重要作用。然而，在哺乳动物中，表达数据主要基于整个组织分析，仍然非常不完整。我们使用寡核苷酸阵列来分析miRNA在小鼠造血系统中的表达。将能够与181miRNAs杂交的互补寡核苷酸固定在膜上，并用来自不同血细胞和神经细胞的低相对分子质量总RNA的放射性标记RNA进行探测。该方法使我们能够分析特定细胞类型的miRNA表达模式，并识别可能对细胞谱系指定和/或细胞效应功能重要的miRNAs。这是在造血系统的细胞中系统分析miRNA基因图谱的第一个报告。正如预期的那样，在造血组和非造血组中，miRNA的表达模式有很大的不同，在造血组中观察到了更细微的差异。有趣的是，在完全分化的效应细胞(Th1和Th2淋巴细胞和肥大细胞)和处于可比分化阶段的前体细胞(双阴性胸腺细胞和前B细胞)之间观察到最明显的相似之处，这表明除了调节特定细胞谱系的承诺过程外，miRNAs可能在细胞分化和维持细胞特性的机制中具有重要的一般作用。
The first report of systematic miRNA profiling in cells of the hematopoietic system suggests that, in addition to regulating commitment to particular cellular lineages, miRNAs might have a general role in cell differentiation and cell identity. MicroRNAs (miRNAs) are a class of recently discovered noncoding RNA genes that post-transcriptionally regulate gene expression. It is becoming clear that miRNAs play an important role in the regulation of gene expression during development. However, in mammals, expression data are principally based on whole tissue analysis and are still very incomplete. We used oligonucleotide arrays to analyze miRNA expression in the murine hematopoietic system. Complementary oligonucleotides capable of hybridizing to 181 miRNAs were immobilized on a membrane and probed with radiolabeled RNA derived from low molecular weight fractions of total RNA from several different hematopoietic and neuronal cells. This method allowed us to analyze cell type-specific patterns of miRNA expression and to identify miRNAs that might be important for cell lineage specification and/or cell effector functions. This is the first report of systematic miRNA gene profiling in cells of the hematopoietic system. As expected, miRNA expression patterns were very different between hematopoietic and non-hematopoietic cells, with further subtle differences observed within the hematopoietic group. Interestingly, the most pronounced similarities were observed among fully differentiated effector cells (Th1 and Th2 lymphocytes and mast cells) and precursors at comparable stages of differentiation (double negative thymocytes and pro-B cells), suggesting that in addition to regulating the process of commitment to particular cellular lineages, miRNAs might have an important general role in the mechanism of cell differentiation and maintenance of cell identity.