Carbohydrate accumulation and sucrose metabolism responses in tomato seedling leaves when subjected to different light qualities
Carbohydrate accumulation and sucrose metabolism responses in tomato seedling leaves when subjected to different light qualities
复制标题
不同光质下番茄幼苗叶片碳水化合物积累及蔗糖代谢反应
DOI：
10.1016/j.scienta.2017.07.053
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发表时间：
2017-11-18
影响因子：
4.3
通讯作者：
Wang, Xiufeng
中科院分区：
文献类型：
--
作者：
Li, Yan;Xin, Guofeng;Wang, Xiufeng
光质被认为影响植物生长发育过程中的许多生理过程。研究光质对番茄生长的影响以番茄（Solanum lycopersicum）幼苗为材料，研究了在相同光合光子通量密度（PPFD）条件下，发光二极管（LED）产生的不同光质对番茄幼苗光合作用的影响，（657 nm，R）、蓝光（457 nm，B）、紫光（417 nm，P）或白色（W），R：B = 1：1，1R1B和R：B = 3：1，3R1B组合对叶片净光合速率（Pn）、生长速率、碳水化合物含量和蔗糖代谢酶活性的影响。结果表明，相对于W，R和B光组合以及单色R光对幼苗株高和茎粗有显著促进作用。而R光和P光下根系生长水平较低，后者显著抑制了幼苗生长和净光合速率。R光处理显著提高了葡萄糖和果糖的含量，R光与B光联合处理显著提高了总糖、淀粉和蔗糖的积累，尤其是3R1 B处理。蔗糖合成酶（SS）活性在不同处理下均有所提高，在3R1B处理下达到最高值，这可能是3R1B处理下淀粉含量显著提高的主要原因。此外，R、B和P光还降低了蔗糖磷酸合成酶（SPS）的活性。R光显著提高了酸性转化酶（AI）和中性转化酶（NI）的活性，而P光则显著降低了它们的活性。研究结果表明，单色R光和组合光3R1B通过影响碳水化合物转化为果糖、葡萄糖、蔗糖和淀粉的代谢来调节植物的形态和光合作用。前者主要是通过提高AI和NI的活性，后者则是通过提高SS和SPS的活性。它们还提高了番茄叶片的最终产品产量，并可能最终提高番茄果实的产量和质量。
Light qualities are thought to affect many plant physiological processes during growth and development. To investigate how light qualities influence tomato (Solanum lycopersicum) seedlings, the present study evaluated the effects of different light qualities generated by light emitting diodes (LEDs) with the same photosynthetic photon flux density (PPFD), including monochromic light red (657 nm, R), blue (457 nm, B), purple (417 nm, P) or white (W), combination of R and B lights (R:B = 1:1, 1R1B and R:B = 3:1, 3R1B) on the net photosynthetic rate (Pn), growth rate, carbohydrate content and sucrose-metabolizing enzyme activities. The results showed that relative to W, the seedling plant height and stem diameter were significantly promoted by combination of R and B lights and monochromic R light. However, the level of root growth was lower under R and P light, and the seedling growth and Pn were significantly suppressed under the latter. Additionally, R light significantly increased the contents of fructose and glucose, and combination of R and B lights significantly enhanced total carbohydrate, starch and sucrose accumulation, especially for 3R1B treatment. Activity of sucrose synthase (SS) was promoted under the different treatments and reached its highest value under 3R1B, which appeared to be a major contributor to the significantly higher content of starch under this treatment. Furthermore, R, B and P light reduced activity of sucrose phosphate synthase (SPS). Activities of acid invertase (AI) and neutral invertase (NI) were significantly increased by R light, but were markedly reduced under P. The results presented here indicated that monochromic R and combination lights 3R1B, could regulate the plant morphology and photosynthesis by the effects on the metabolism of carbohydrate into fructose, glucose, sucrose and starch, mainly through the enhanced activities of AI and NI under the former treatment, while SS and SPS in the latter treatment, respectively. They also improved the end-product output in tomato leaves, and may ultimately improve the yield and quality of tomato fruit.