Effect of macromolecular crowding on DNA:Au nanoparticle bioconjugate assembly
Effect of macromolecular crowding on DNA:Au nanoparticle bioconjugate assembly
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DOI：
10.1021/la048434l
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发表时间：
2004-11-09
期刊：
影响因子：
3.9
通讯作者：
Keating, CD
中科院分区：
文献类型：
--
作者：
Goodrich, GP;Helfrich, MR;Keating, CD
DNA：Au纳米球生物结合物在生物传感和自下而上的材料组装中有应用。当加入互补的DNA寡核苷酸时，这些生物结合物可以选择性地组装成三维聚集体，并可以通过在DNA双链变性的熔融转变温度以上加热来解离。在这里，我们描述了聚合物溶质对DNA：Au纳米粒子生物偶联组件热变性行为的影响。聚合物溶质可以通过大分子拥挤显著影响生化反应。以不同相对分子质量的聚乙二醇、S(PEGS)和右旋糖苷为拥挤剂。虽然聚乙二醇和葡聚糖都提高了DNA：Au聚集体的稳定性，但聚乙二醇组分的熔融转变温度受到的影响更大。聚合物的相对分子质量不如聚合物化学和溶液中的重量百分比重要。对于高(15%)重量百分比的聚乙二醇组分，即使在没有互补寡核苷酸的情况下也能观察到聚集。这些结果强调了在大分子拥挤中除了物理体积排除之外聚合物化学的重要性，并指出在设计基于生物识别的纳米颗粒在复杂基质(即任何涉及聚合物溶质)中的组装方案时了解这些影响的重要性。
DNA:Au nanosphere bioconjugates have applications in biosensing and in the bottom-up assembly of materials. These bioconjugates can be selectively assembled into three-dimensional aggregates upon addition of complementary DNA oligonucleotides and can be dissociated by heating above a melting transition temperature at which the DNA duplexes are denatured. Herein we describe the impact of polymeric solutes on the thermal denaturation behavior of DNA:Au nanoparticle bioconjugate assemblies. Polymeric solutes can dramatically impact biochemical reactions via macromolecular crowding. Poly(ethylene glycol)s (PEGs) and dextrans of varying molecular weights were used as crowding reagents. While both PEG and dextran increased the stability of DNA:Au aggregates, melting transition temperatures in the presence of PEG were impacted more significantly. Polymer molecular weight was less important than polymer chemistry and weight percent in solution. For a high (15%) weight percent of PEG, aggregation was observed even in the absence of complementary oligonucleotides. These results underscore the importance of polymer chemistry in addition to physical volume exclusion in macromolecular crowding and point to the importance of understanding these effects when designing biorecognition-based nanoparticle assembly schemes in complex matrixes (i.e., any involving polymeric solutes).