IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement.
IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement.
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DOI：
10.4049/jimmunol.125.6.2665
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发表时间：
1980-12
影响因子：
4.4
通讯作者：
M. Sarmiento;A. Glasebrook;F. Fitch
中科院分区：
文献类型：
--
作者：
M. Sarmiento;A. Glasebrook;F. Fitch
已经衍生出五种大鼠单克隆抗体，其表达对承载Lyt 2抗原的分子复合物上存在的决定簇的特异性。当在非还原和还原条件下分析时，由这些抗体中的每一种沉淀的125 I标记的多肽的SDS-聚丙烯酰胺凝胶电泳分别显示3种组分（150，000、75，000和33，000道尔顿）和2种组分（44，000和33，000道尔顿）。其中两种抗体是IgG，对Lyt 2.2决定簇具有特异性;另外3种是IgM，与Lyt 2.2以外的决定簇反应，这些决定簇是非多态性的。5种抗体中的每一种都可以阻断5天MLC细胞或克隆的溶细胞性T细胞在不存在C.用任何这些抗体和C处理应答脾细胞抑制MLC中细胞溶解活性的产生。
Five rat monoclonal antibodies have been derived that express specificities for determinants present on the molecular complex bearing the Lyt 2 antigen. SDS-polyacrylamide gel electrophoresis of 125I-labeled polypeptides precipitated by each of these antibodies reveal 3 components (150,000, 75,000, and 33,000 daltons), and 2 components (44,000 and 33,000 daltons) when analyzed under nonreducing and reducing conditions, respectively. Two of these antibodies are IgG and are specific for the Lyt 2.2 determinant; the other 3 are IgM and react with determinants other than Lyt 2.2, which are nonpolymorphic. Each of the 5 antibodies can block the cytolytic activities of 5-day MLC cells or of cloned cytolytic T cells in the absence of C. Treatment of responding spleen cells with any of these antibodies and C inhibits the generation of cytolytic activity in MLC.