Location of transient ectodermal progenitor potential in mouse development
Location of transient ectodermal progenitor potential in mouse development
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DOI：
10.1242/dev.092866
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发表时间：
2013-11-15
期刊：
影响因子：
4.6
通讯作者：
Rossant, Janet
中科院分区：
文献类型：
--
作者：
Li, Lingyu;Liu, Chang;Rossant, Janet
外胚层是小鼠早期胚胎的三个典型胚层之一，具有发育成中枢神经系统和表皮的能力。由于早期外胚层是一个短暂的发育阶段，分子标记很少，因此它是小鼠胚胎发育中最不为人所知的种系层。在这项工作中，我们研究了不同发育阶段（E6.5，E7.0和E7.5）的分离外胚层组织响应BMP信号的分化潜力，并确定了E7.0时胚胎前近端侧的一个瞬时区域，该区域具有分别响应BMP 4的存在或缺失而成为神经或表皮外胚层的能力。此外，我们证明了抑制Nodal信号传导可以在体外外植体分化过程中将多能E6.5外胚层细胞导向外胚层谱系。我们的工作不仅提高了我们对早期胚胎外胚层发育的理解，而且为外胚层组织的再生分化提供了一个框架。
Ectoderm is one of the three classic germ layers in the early mouse embryo, with the capacity to develop into both the central nervous system and epidermis. Because it is a transient phase of development with few molecular markers, the early ectoderm is the least understood germ layer in mouse embryonic development. In this work, we studied the differentiation potential of isolated ectoderm tissue in response to BMP signaling at various developmental stages ( E6.5, E7.0 and E7.5), and identified a transient region in the anteriorproximal side of the embryo at E7.0 that possesses the ability to become neural or epidermal ectoderm in response to the absence or presence of BMP4, respectively. Furthermore, we demonstrated that inhibition of Nodal signaling could direct the pluripotent E6.5 epiblast cells towards ectoderm lineages during differentiation in explants in vitro. Our work not only improves our understanding of ectodermal layer development in early embryos, but also provides a framework for regenerative differentiation towards ectodermal tissues.