Comparative study of non-porous anion-exchange chromatography, capillary gel electrophoresis and capillary electrophoresis in polymer solutions in the separation of DNA restriction fragments
Comparative study of non-porous anion-exchange chromatography, capillary gel electrophoresis and capillary electrophoresis in polymer solutions in the separation of DNA restriction fragments
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无孔阴离子交换层析、毛细管凝胶电泳和聚合物溶液毛细管电泳分离DNA限制性片段的对比研究
DOI：
10.1016/0021-9673(94)85197-2
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发表时间：
1994
影响因子：
4.1
通讯作者：
M. Tsuhako
中科院分区：
文献类型：
--
作者：
C. Sumita;Y. Baba;Kayoko Hide;N. Ishimaru;K. Samata;A. Tanaka;M. Tsuhako
HPLC使用100 mm柱填充无孔阴离子交换剂得到高分辨率分离的DNA限制性片段高达8000碱基对（bp）。利用毛细管凝胶电泳（CGE）和毛细管电泳（CE）在缠结聚合物溶液中实现了高达12 000 bp的DNA限制性片段的高分辨率分离。这些方法进行了比较，就其性能和效率的DNA限制性片段的分辨率。CGE的分辨率高于其他技术。使用HPLC实现了高达500 bp的DNA片段的50-100 bp分辨率，在缠结聚合物溶液中使用CE实现了50 bp分辨率，并且使用CGE实现了10 bp分辨率。在7 ·105塔板/m的聚合物溶液中，用3 ·106塔板/m的CGE获得的塔板数高于1 ·106塔板/m的HPLC和CE。讨论了高效液相色谱和毛细管电泳技术的优点和局限性。
HPLC using a 100-mm column packed with a non-porous anion exchanger gave high-resolution separations of DNA restriction fragments up to 8000 base pairs (bp). High-resolution separation of DNA restriction fragments up to 12 000 bp was achieved using capillary gel electrophoresis (CGE) and capillary electrophoresis (CE) in entangled polymer solutions. These methods were compared with respect to their performance and efficiencies in the resolution of DNA restriction fragments. The resolving power of CGE is higher than those of the other techniques. The 50–100 bp resolution of DNA fragments up to 500 bp was realized using HPLC, 50 bp resolution using CE in entangled polymer solutions and 10 bp resolution using CGE. The plate number that was achieved with CGE of 3 · 106plates/m was higher than those of HPLC of 1 · 106and CE in polymer solutions of 7 · 105plates/m. The advantages and limitations of HPLC and capillary electrophoretic techniques are discussed.