Antimicrobial Effects of Drug-Containing Electrospun Matrices on Osteomyelitis-Associated Pathogens
Antimicrobial Effects of Drug-Containing Electrospun Matrices on Osteomyelitis-Associated Pathogens
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DOI：
10.1016/j.joms.2014.01.007
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发表时间：
2014-07-01
影响因子：
1.9
通讯作者：
Bottino, Marco C.
中科院分区：
文献类型：
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作者：
Waeiss, Robert A.;Negrini, Thais C.;Bottino, Marco C.
目的：合成基于聚对二氧六环酮（PDS）的药物输送系统材料和方法：PDS基质采用静电纺丝法制备，药物掺入如下：（G）组1，5wt%VANC; G2，10wt%VANC; G3，5wt%RIF; G4，10wt%RIF; G5，5wt%VANC+RIF;和G6，10wt%VANC + RIF。纯PDS的对照组也进行静电纺丝（G7）。金黄色葡萄球菌和葡萄球菌形成的生物膜。表皮细胞在电纺基质上生长24小时。在生物膜形成后评估活细胞的计数。结果：G5、G6和纯PDS（G7）的平均活细胞数分别最低和最高（P
Purpose: To synthesize polydioxanone (PDS)-based drug delivery systems (hereafter referred to as "matrices'') containing vancomycin (VANC) and/or rifampicin (RIF) and investigate their effect on the inhibition of biofilm growth containing osteomyelitis (OM)-associated pathogens.Materials and Methods: PDS matrices were prepared by electrostatic spinning, and the drugs were incorporated as follows: group (G) 1, 5wt%VANC; G2, 10wt% \VANC; G3, 5wt%RIF; G4, 10wt%RIF; G5, 5wt%VANC+RIF; and G6, 10wt% VANC+ RIF. A control group of pure PDS was also electrospun (G7). Biofilms formed by Staphylococcus aureus and S. epidermidis were grown on the electrospun matrices for 24 hours. The counts of viable cells were assessed after biofilm formation. The fiber morphology and biofilms were imaged using a scanning electron microscope.Results: G5 and G6 and pure PDS (G7) had the lowest and highest mean number of viable cell counts, respectively (P