The 'sniffer-patch' technique for detection of neurotransmitter release
The 'sniffer-patch' technique for detection of neurotransmitter release
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DOI：
10.1016/s0166-2236(96)01039-9
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发表时间：
1997-05-01
影响因子：
15.9
通讯作者：
Allen, TGJ
中科院分区：
文献类型：
--
作者：
Allen, TGJ
已采用多种技术来检测和测量生物制品中神经递质的释放。虽然许多这些方法提供了令人印象深刻的灵敏度，但很少有能够将灵敏度与研究单细胞量子释放所需的时间酸空间分辨率结合起来。一种重新引起人们兴趣并有潜力填补这一空白的检测方法是所谓的“嗅探器补丁”技术。在本文中，讨论了使用该技术的实际方面的具体示例，以及校准这些生物传感器以扩展其应用以提供量子发射器释放的定量（除了简单的定性）测量所涉及的程序。
A wide variety of techniques have been employed for the detection and measurement of neurotransmitter release from biological preparations. Whilst many of these methods offer impressive levels of sensitivity, few are able to combine sensitivity with the necessary temporal acid spatial resolution required to study quantal release from single cells. One detection method that is seeing a revival of interest and has the potential to fill this niche is the so-called 'sniffer-patch' technique. In this article, specific examples of the practical aspects of using this technique are discussed along with the procedures involved in calibrating these biosensors to extend their applications to provide quantitative, in addition to simple qualitative, measurements of quantal transmitter release.