Bioreactor systems for tissue engineering: A four-dimensional challenge
Bioreactor systems for tissue engineering: A four-dimensional challenge
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DOI：
10.1007/1-4020-3741-4_1
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发表时间：
2005-01-01
期刊：
影响因子：
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通讯作者：
Chaudhuri, J. B.
中科院分区：
文献类型：
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作者：
Ellis, M.;Jarman-Smith, M.;Chaudhuri, J. B.
在体外创建三维组织将需要良好控制的培养工具，以最大限度地提高营养物质的转移，允许多种细胞类型的培养，并断言对细胞的机械力。生物反应器技术的发展将在这方面大有帮助。虽然仍处于起步阶段，但我们可以整合一些基本的设计规则以及一般的生物学和物理学考虑，以创建生物反应器系统，该系统将管理组织中存在的单个细胞之间，细胞与基质之间以及细胞与其环境之间的复杂相互作用。此外，还变得清楚的是，单一生物反应器类型将不适合于生长所有组织类型。定制的生物反应器系统将需要用于特定的组织或组织类别。本章没有详细介绍与组织工程生物反应器相关的所有问题;其目的是概述有助于开发用于人体组织生长的动态培养系统的关键特征。本书的以下章节提供了生物反应器设计和操作的不同方面的知识来源，我们希望这些知识将为组织工程的未来成功发展提供基础。
ConclusionsThe in vitro creation of three-dimensional tissues will require well-controlled culture tools to maximise nutrient mass transfer, allow the culture of multiple cell types, and assert mechanical forces on the cells. The development of bioreactor technologies will help greatly in this respect. Although still in its infancy, there are some basic design rules and general biological and physical considerations that we can integrate to create bioreactor systems that will manage the complex interactions that exist in tissue between individual cells, between cells and the matrix and cells and their environment. Furthermore, it is also becoming clear that a single bioreactor type will not be suitable to grow all tissue types. Bespoke bioreactor systems will be required for specific tissues or classes of tissue. This chapter has not detailed all of the issues related to tissue engineering bioreactors; its aim was to outline the key features that will contribute to the development of dynamic culture systems for the growth of human tissues. The following chapters in this book provide a source of knowledge on different aspects of bioreactor design and operation that we hope will provide a foundation for the future successful development of tissue engineering.