Long-term ovarian function in sheep after ovariectomy and transplantation of autografts stored at -196 C
Long-term ovarian function in sheep after ovariectomy and transplantation of autografts stored at -196 C
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DOI：
10.1210/en.140.1.462
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发表时间：
1999-01-01
期刊：
影响因子：
4.8
通讯作者：
Gosden, RG
中科院分区：
文献类型：
--
作者：
Baird, DT;Webb, R;Gosden, RG
我们以前已经证明，卵巢功能和生育能力可以保存在羊去势后，自体移植冷冻保存的卵巢皮质条。在目前的实验中，我们通过详细测量自体移植后近2年的卵巢功能来研究这种移植物的长期存活。在卵巢切除术和冷冻/解冻移植物移植后，FSH和LH的浓度升高至去势水平约14周，然后逐渐下降至约60周时接近正常水平。1994年10月至1995年3月的繁殖季节，所有母羊都有5-10个发情周期，其长度与4只对照母羊相似。黄体功能所示的孕酮浓度是相同的移植前和11个月后。相反，FSH和LH的基础浓度在整个黄体期持续升高，但在卵泡期正常下降。在移植后22个月的实验结束时测量的卵巢静脉血浆中的白蛋白A浓度显著低于对照母羊（平均值+/- SE，409 +/- 118 vs. 1914 +/- 555 pg/ml; P < 0.004）。将冷冻/解冻的卵巢组织移植到SCID小鼠体内，结果表明约28%的原始卵泡存活。所有移植到绵羊体内的卵巢都含有大的有腔卵泡和/或囊肿，但在22个月后尸检时回收的原始卵母细胞很少。这些结果表明，尽管原始卵泡的总数急剧减少，绵羊自体移植冷冻/非冷冻卵巢后的周期性卵巢功能得以保留解冻的卵巢组织，并提供实验证实，这种技术可以提供一种手段，以保持妇女的生育能力，接受化疗或放疗的恶性疾病。
We have previously demonstrated that ovarian function and fertility can be preserved in sheep after castration by autotransplantation of cryopreserved strips of ovarian cortex. In the current experiments we have investigated the long term survival of such grafts by detailed measurements of ovarian function for a period of nearly 2 yr after autotransplantation. After ovariectomy and transplantation of frozen/thawed grafts, the concentrations of FSH and LH rose to castrate levels for about 14 weeks before falling gradually to reach near-normal levels at about 60 weeks. In the breeding season From October 1994 to March 1995, all ewes had 5-10 estrous cycles that were similar in length to those in the 4 control ewes. Luteal function as indicated by the progesterone concentration was identical before and 11 months after transplantation. In contrast, the basal concentrations of FSH and LH were persistently raised throughout the luteal phase, but showed a normal decline during the follicular phase. The concentration of inhibin A in ovarian venous plasma measured at the end of the experiment 22 months after transplantation was significantly lower than that in control ewes (mean +/- SE, 409 +/- 118 vs. 1914 +/- 555 pg/ml; P < 0.004). Transplantation of frozen/thawed ovarian tissue to SCID mice demonstrated that about 28% of primordial follicles survived the procedure. All of the ovaries transplanted into sheep contained large antral follicles and/or cysts, but very few primordial oocytes when recovered at autopsy after 22 months.These results demonstrate that despite a drastic reduction in the total number of primordial follicles, cyclical ovarian function is preserved in sheep after autotransplantation of frozen/thawed ovarian tissue and provide experimental confirmation that such a technique could provide a means of preserving fertility in women undergoing chemo- or radiotherapy for malignant disease.