Kupffer cells support extramedullary erythropoiesis induced by nitrogen-containing bisphosphonate in splenectomized mice
Kupffer cells support extramedullary erythropoiesis induced by nitrogen-containing bisphosphonate in splenectomized mice
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DOI：
10.1016/j.cellimm.2011.06.025
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发表时间：
2011-01-01
影响因子：
4.3
通讯作者：
Nakamura, Masanori
中科院分区：
文献类型：
--
作者：
Otsuka, Hirotada;Yagi, Hideki;Nakamura, Masanori
我们以前的研究表明，注射含氮双膦酸盐（NBP）诱导红细胞生成的网站从骨髓（BM）转移到脾脏。这是由于支持红细胞生成的BM驻留巨噬细胞的消耗。在这项研究中，我们研究了NBP治疗诱导的脾切除小鼠髓外造血，重点是肝造血。NBP处理的小鼠没有表现出贫血或促红细胞生成素产生的显着变化，而巨核细胞和红细胞生成不断观察到在肝脏中。在窦腔中检测到成红细胞岛。Kupffer细胞在NBP处理后表达VCAM-1，这是成红细胞分化的重要因素。Cl 2 MBP-脂质体处理耗尽成红细胞岛，并减少肝脏中造血细胞的数量，如通过集落形成测定所确定的。总之，这些结果表明枯否细胞支持红细胞生成，在肝脏中充当基质细胞，并且它们可能在紧急情况下充当造血前体细胞的小生境。(C)2011 Elsevier Inc. All rights reserved.
Our previous study indicated that injecting nitrogen-containing bisphosphonate (NBP) induced the site of erythropoiesis to shift from the bone marrow (BM) to the spleen. This was due to the depletion of BM-resident macrophages, which support erythropoiesis. In this study, we examined NBP treatment-induced extramedullary hematopoiesis in splenectomized mice, focusing on hepatic hematopoiesis. NBP-treated mice did not display anemia or significant change in erythropoietin production, while megakaryopoiesis and erythropoiesis were constantly observed in the liver. Erythroblastic islands were detected in the sinusoidal lumen. Kupffer cells expressed VCAM-1 following NBP treatment, which is an important factor for erythroblast differentiation. Cl2MBP-liposome treatment depleted the erythroblastic islands, and decreased the number of hematopoietic cells in the liver, as determined by colony forming assays. Together, these results indicate that Kupffer cells support erythropoiesis, acting as stromal cells in the liver, and that they might act as a niche for hematopoietic precursor cells in an emergency. (C) 2011 Elsevier Inc. All rights reserved.