Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans
Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans
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DOI：
10.1006/dbio.1999.9470
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发表时间：
1999-11-15
影响因子：
2.7
通讯作者：
Sengupta, P
中科院分区：
文献类型：
--
作者：
Miyabayashi, T;Palfreyman, MT;Sengupta, P
核受体(NRs)是一大类由配体调节的转录调节因子，已被证明在许多发育过程中发挥作用。秀丽线虫基因组被预测编码一个大的和不同的NR蛋白家族。这些基因中的大多数功能尚不清楚。作为确定它们角色的第一步，我们已经开始了对这些基因的子集的表达和功能调查。在这项研究中，我们展示了28个NR基因中的21个的表达，表明很大一部分预测基因可能编码功能基因产物。我们发现有五个基因主要在神经细胞中表达，其他基因在多种细胞类型中表达。有趣的是，我们发现有8个基因仅在外侧皮下(Seam)细胞中表达。从NRS的序列分析得出，这8个基因在相邻连接树中具有高度的同源性和聚集性，表明它们是由来自共同祖先的基因复制而产生的。我们发现，这个亚家族的三个成员中的每一个过表达都会导致类似的发育缺陷，这与这些基因在外侧皮下细胞功能中的多余作用是一致的。(C)1999年学术出版社。
Nuclear receptors (NRs) are a large class of ligand-regulated transcriptional modulators that have been shown to play roles in many developmental processes. The Caenorhabditis elegans genome is predicted to encode a large and divergent family of NR proteins. The functions of most of these genes are unknown. As a first step toward defining their roles, we have initiated an expression and functional survey of a subset of these genes. In this study, we demonstrate expression of 21 of 28 NR genes examined, indicating that a large fraction of the predicted genes likely encode functional gene products. We show that five genes are expressed predominantly in neuronal cells, while others are expressed in multiple cell types. Interestingly, we find that eight genes are expressed exclusively in the lateral hypodermal (seam) cells. These eight genes share a high degree of overall homology and cluster in a neighbor-joining tree derived from sequence analysis of the NRs, suggesting that they arose by gene duplication from a common ancestor. We show that overexpression of each of three members of this subfamily results in similar developmental defects, consistent with a redundant role for these genes in the function of the lateral hypodermal cells. (C) 1999 Academic Press.