Synaptic transmission of neural stem cells seeded in 3-dimensional PLGA scaffolds.
Synaptic transmission of neural stem cells seeded in 3-dimensional PLGA scaffolds.
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DOI：
10.1016/j.biomaterials.2009.03.046
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发表时间：
2009-08
期刊：
影响因子：
14
通讯作者：
Y. Xiong;Yuan-shan Zeng;Chen-guang Zeng;Bao-ling Du;Liu-min He;D. Quan;Wei Zhang;Jun-mei Wang
中科院分区：
文献类型：
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作者：
Y. Xiong;Yuan-shan Zeng;Chen-guang Zeng;Bao-ling Du;Liu-min He;D. Quan;Wei Zhang;Jun-mei Wang
为了探索工程化神经组织的治疗潜力，我们将遗传修饰的神经干细胞（NSCs）和聚（乳酸-共-乙醇酸）（PLGA）聚合物结合以在体外产生人工神经网络。将转染NT-3或其受体TrkC基因的NSC接种到PLGA支架中。培养14 d后，神经干细胞在支架内分布广泛，存活良好。在>70%的这些移植细胞中检测到针对Map 2的免疫反应性，表明向神经元的分化率高。突触蛋白-I和PSD 95的免疫组化显示突触结构的形成，这也在电子显微镜下观察。此外，使用FM 1 -43动态成像，发现这些分化的神经元中的突触是可兴奋的并且能够释放突触囊泡。总之，我们的人工PLGA构建体允许NSC向神经元分化，建立连接并表现出突触活动。这些发现为将来这种人工构建体在神经修复中的应用或移植提供了生物学基础。
To explore therapeutic potential of engineered neural tissue, we combined genetically modified neural stem cells (NSCs) and poly(lactic acid-co-glycolic acid) (PLGA) polymers to generate an artificial neural network in vitro. NSCs transfected with either NT-3 or its receptor TrkC gene were seeded into PLGA scaffold. The NSCs were widely distributed and viable in the scaffold after culturing for 14 days. Immunoreactivity against Map2 was detected in >70% of these grafted cells, suggesting a high rate of differentiation toward neurons. Immunostaining of synapsin-I and PSD95 revealed formation of synaptic structures, which was also observed under electron microscope. Furthermore, using FM1-43 dynamic imaging, synapses in these differentiated neurons were found to be excitable and capable of releasing synaptic vesicles. Taken together, our artificial PLGA construct permits NSCs to differentiate toward neurons, establish connections and exhibit synaptic activities. These findings provide a biological basis for future application or transplantation of this artificial construct in neural repair.