Aldosterone stimulates K secretion prior to onset of Na absorption in guinea pig distal colon.
Aldosterone stimulates K secretion prior to onset of Na absorption in guinea pig distal colon.
复制标题
醛固酮在豚鼠远端结肠开始吸收钠之前刺激钾分泌。
DOI：
10.1152/ajpcell.1994.266.2.c552
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Halm,ST
中科院分区：
文献类型：
--
作者：
Halm,DR;Halm,ST
用醛固酮刺激离体豚鼠远端结肠，在Ussing室中测量短路电流（Isc）和组织电导（Gt）。对醛固酮的反应延迟约20分钟，并导致Isc呈阴性，与K分泌一致。约1小时后，Isc开始增加，并最终变为阳性，与随后的Na吸收刺激一致。粘膜阿米洛利可抑制钠吸收反应，而不改变钾分泌速率。同样，布美他尼可抑制钾分泌而不改变钠吸收。在螺内酯，放线菌素D，放线菌酮或放线菌酮的存在下，醛固酮未能刺激钾分泌和钠吸收。对醛固酮的剂量反应提供了2.6 +/- 0.5 nM的表观Kd，与与这种分泌反应偶联的高亲和力受体一致。刺激的K分泌素肾上腺素没有产生一个附加的增加K分泌，这表明相同的细胞类型响应于醛固酮和肾上腺素和醛固酮诱导的蛋白质不是一个膜蛋白负责K分泌。
Distal colon from guinea pig was stimulated in vitro by aldosterone in Ussing chambers that allowed measurement of short-circuit current (Isc) and tissue conductance (Gt). The response to aldosterone was delayed by approximately 20 min and resulted in a negative Isc, consistent with K secretion. Approximately 1 h later the Isc began to increase and eventually became positive, consistent with subsequent stimulation of Na absorption. The Na-absorptive response could be inhibited by mucosal amiloride without altering the rate of K secretion. Similarly, K secretion could be inhibited by serosal bumetanide without altering Na absorption. In the presence of spironolactone, actinomycin D, or cycloheximide, aldosterone failed to stimulate both K secretion and Na absorption. A dose response to aldosterone provided an apparent Kd of 2.6 +/- 0.5 nM, consistent with a high-affinity receptor coupled to this secretory response. Stimulation by the K secretagogue epinephrine did not produce an additive increase in K secretion, suggesting that the same cell type responds to both aldosterone and epinephrine and that the protein induced by aldosterone was not one of the membrane proteins responsible for K secretion.
大鼠唾液腺和远端结肠中 I 型皮质类固醇受体样免疫反应性：皮质类固醇的调节
影响因子：
4.1
作者：
Z. Krozowski;K. Wendell;R. Ahima;R. Harlan
通讯作者：
R. Harlan
醛固酮激活两栖动物肾脏靶细胞中的 Na /H 交换并提高细胞质 pH 值。
DOI：
--
发表时间：
1987
影响因子：
11.1
作者：
H. Oberleithner;M. Weigt;H. Westphale;W. Wang
通讯作者：
W. Wang
醛固酮在靶上皮细胞中的作用。
影响因子：
--
作者：
D. Marver
通讯作者：
D. Marver