Architecture of nucleotide excision repair complexes: DNA is wrapped by UvrB before and after damage recognition
Architecture of nucleotide excision repair complexes: DNA is wrapped by UvrB before and after damage recognition
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DOI：
10.1093/emboj/20.3.601
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发表时间：
2001-02-01
期刊：
影响因子：
11.4
通讯作者：
Goosen, N
中科院分区：
文献类型：
--
作者：
Verhoeven, EEA;Wyman, C;Goosen, N
核苷酸切除修复（NER）是一种主要的DNA修复机制，可以识别广泛的DNA损伤。在大肠杆菌中，NER中的损伤识别是由UvrA和UvrB蛋白完成的。我们用原子力显微镜分析了由UvrA、UvrB和（损伤的）DNA形成的不同蛋白-DNA复合物的结构特性。对UvrA（2）B复合物进行分析以寻找损伤，发现DNA包裹在UvrB蛋白周围，包含约7个螺旋圈的区域。在UVrB-DNA切割前复合物中，DNA以类似的方式包裹，并且这种DNA构型依赖于ATP结合。在此基础上，提出了DNA包裹在损伤识别中的作用。有证据表明，DNA包裹在切口前复杂的也刺激率的切口UvrC。
Nucleotide excision repair (NER) is a major DNA repair mechanism that recognizes a broad range of DNA damages. In Escherichia coli, damage recognition in NER is accomplished by the UvrA and UvrB proteins, We have analysed the structural properties of the different protein-DNA complexes formed by UvrA, UvrB and (damaged) DNA using atomic force microscopy. Analysis of the UvrA(2)B complex in search of damage revealed the DNA to be wrapped around the UvrB protein, comprising a region of about seven helical turns. In the UVrB-DNA pre-incision complex the DNA is wrapped in a similar way and this DNA configuration is dependent on ATP binding. Based on these results, a role for DNA wrapping in damage recognition is proposed. Evidence is presented that DNA wrapping in the pre-incision complex also stimulates the rate of incision by UvrC.