Resection of Scar Tissue in Rats With Spinal Cord Injury Can Promote the Expression of βⅢ-tubulin in the Injured Area
Resection of Scar Tissue in Rats With Spinal Cord Injury Can Promote the Expression of βⅢ-tubulin in the Injured Area
复制标题
脊髓损伤大鼠疤痕组织切除可促进损伤区βⅢ-微管蛋白的表达
DOI：
10.1016/j.wneu.2022.10.069
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通讯作者：
Dejun Sun
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作者：
Baoguo Liu;Guoqing Liu;Changyang Li;Sumei Liu;Dejun Sun
研究背景以往的研究表明，脊髓损伤后损伤部位形成的瘢痕组织阻碍了神经再生和功能恢复。然而，这些研究人员试图阻止脊髓损伤后瘢痕的形成以促进神经再生，但这与他们的愿望背道而驰，表明瘢痕的形成可能在脊髓损伤后的功能恢复中发挥作用。方法在T8-T9脊髓横断伤大鼠模型的基础上，选择不同的关键时间点切除脊髓损伤后形成的瘢痕组织，以探讨瘢痕形成在脊髓损伤后功能修复中的作用。首先通过Basso Beattie Bresnahan评分和电生理检查评价运动功能的恢复情况；其次，主要通过免疫荧光βⅢ-微管蛋白染色分析功能恢复的病理特征；最后，通过高通量测序分析与运动功能恢复相关的基因。结果免疫荧光结果显示，脊髓损伤后第2周，瘢痕组织切除明显促进了损伤区域运动功能的恢复和βⅢ-微管蛋白的表达。此外，RNA-seq研究表明，早期切除前后组织中TUBB3和Tubb6基因的表达及其他神经再生途径有显著差异。结论脊髓损伤后第2周切除瘢痕组织可促进神经再生。TUBB3和Tubb6基因可能是本研究中脊髓损伤治疗的潜在靶点。
BackgroundPrevious research shows that scar tissue formed in the injured area after spinal cord injury blocks nerve regeneration and functional recovery. However, those researchers tried to prevent the formation of scar after spinal cord injury to promote nerve regeneration, but it ran counter to their desire, indicating that the formation of scar might play a role in functional recovery after spinal cord injury.MethodsTo investigate roles of scar formation on functional repair after spinal cord injury, we selected several different key time points to resect the scar tissue formed after spinal cord injury based on the rat models of the T8-T9 transection injury of spinal cord. First, the recovery of motor function was evaluated by Basso Beattie Bresnahan score and electrophysiologic examination; second, the pathologic features of functional recovery were analyzed mainly by immunofluorescence βⅢ-tubulin staining; finally, the genes related to the recovery of motor function were predicted by high-throughput sequencing analysis.ResultsImmunofluorescence results showed that the resection of scar tissue promoted significantly the recovery of motor function and the expression of βⅢ-tubulin in the injured area in the second week after spinal cord injury. Furthermore, RNA-seq studies showed that Tubb3 and Tubb6 gene expression and other neural regeneration pathways were significantly different in the tissue before and after early resection.ConclusionsExcision of scar tissue in the second week promoted nerve regeneration after spinal cord injury. Tubb3 and Tubb6 genes might be the potential targets for spinal cord injury therapy in our study.