Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion
Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion
复制标题
羊栖菜岩藻依聚糖组分的理化特性及其对 P-选择素介导的细胞粘附的拮抗作用
DOI：
10.1016/j.ijbiomac.2019.03.218
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发表时间：
2019
影响因子：
8.2
通讯作者：
Haibin Tong
中科院分区：
文献类型：
--
作者：
Siya Wu;Xu Zhang;Jian Liu;Jianxi Song;Ping Yu;Peichao Chen;Zhiyong Liao;Mingjiang Wu;Haibin Tong
P-选择素介导内皮细胞和中性粒细胞之间的粘附，是治疗急性炎症相关疾病的一个有前途的靶点。据报道，褐藻岩藻多糖可以拮抗P-选择素的功能。然而，对羊栖菜褐藻糖胶的分级分离、理化性质的研究以及具有P-选择素拮抗活性的褐藻糖胶组分的筛选尚未见报道。本研究系统地分离和分级了S.通过离子交换层析和尺寸排阻层析对梭形岩藻依聚糖进行纯化，得到8个岩藻依聚糖级分。采用化学方法、HPLC和FT-IR对岩藻聚糖硫酸酯组分的理化性质进行了表征，采用静态粘附实验和平行板流动室法对岩藻聚糖硫酸酯组分抑制P-选择素介导的白细胞粘附的能力进行了评价。结果表明，岩藻聚糖硫酸酯组分具有不同的理化性质，包括总糖、糖醛酸和硫酸根含量、分子量和单糖组成。在所有的岩藻依聚糖组分中，SFF-32和SFF-42对P-选择素介导的细胞粘附显示出更好的阻断能力。
P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases. It is reported that brown algal fucoidans can antagonize P-selectin function. However, the fractionation and physicochemical characterization ofSargassumfusiformefucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated. In this study, we isolated and fractionated systematically theS. fusiformefucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions. Their physicochemical characterization was determined by chemical methods, HPLC and FT-IR. The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber. Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions. Among all the fucoidan fractions, SFF-32 and SFF-42 showed better blocking ability against P-selectin-mediated cell adhesion.