INTERFERON TREATMENT INHIBITS ONSET OF HERPES-SIMPLEX VIRUS IMMEDIATE-EARLY TRANSCRIPTION
INTERFERON TREATMENT INHIBITS ONSET OF HERPES-SIMPLEX VIRUS IMMEDIATE-EARLY TRANSCRIPTION
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DOI：
10.1016/0042-6822(88)90637-x
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发表时间：
1988-05-01
期刊：
影响因子：
3.7
通讯作者：
JACOBSEN, H
中科院分区：
文献类型：
--
作者：
MITTNACHT, S;STRAUB, P;JACOBSEN, H
用鼠IFN-α预处理小鼠脾巨噬细胞的原代培养物β的导致1型单纯疱疹病毒的稳定抑制。病毒DNA、RNA和蛋白质合成的分析鉴定出“立即早期”基因的表达是IFN介导的抑制的主要靶点。感染后早期细胞核中病毒DNA的测定，即在DNA复制开始之前，表明在IFN预处理的巨噬细胞中，病毒摄取、转运到细胞核和DNA稳定性没有降低。核径流转录分析显示IFN处理后立即早期转录率显着降低。ICP 4基因的末端特异性探针定位转录起始的抑制。北方印迹分析揭示了与所观察到的转录抑制一致的ICP 4转录物的减少。观察到的早期基因转录的抑制可能是立即早期基因表达减少的结果。
Pretreatment of primary cultures of splenic mouse macrophages with murine IFN-.alpha./.beta. leads to a stable inhibition of herpes simplex virus type 1. Analysis of viral DNA, RNA, and protein synthesis identifies expression of "immediate-early" genes as a major target of IFN-mediated inhibition. Determination of viral DNA in the nuclei early after infection, i.e., before onset of DNA replication, suggests that virus uptake, transport to the nucleus, and DNA stability are not decreased in IFN-pretreated macrophages. Nuclear runoff transcription analysis shows a significant reduction of immediate-early transcription rates following IFN treatment. End-specific probes for the ICP4 gene locate the inhibition to the onset of transcription. Northern blot analysis reveals a decrease in ICP4 transcripts in accordance with the observed inhibition of transcription. The observed inhibition of early gene transcription may be a consequence of decreased immediate-early gene expression.