Heterogeneity of the induction of HLA-DR expression by human immune interferon on glioma cell lines and their clones.
Heterogeneity of the induction of HLA-DR expression by human immune interferon on glioma cell lines and their clones.
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人免疫干扰素对神经胶质瘤细胞系及其克隆诱导 HLA-DR 表达的异质性。
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发表时间：
1986
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通讯作者：
Nicolas Tribolet de
中科院分区：
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作者：
V. Piguet;S. Carrel;A. Diserens;J. Mach;Nicolas Tribolet de
研究了人重组免疫干扰素（ifn - γ）对10个恶性胶质瘤细胞系、8个由这些细胞系衍生的克隆或亚克隆以及一个胎儿星形胶质细胞系对HLA-DR和HLA-A、-B和-C的调节作用。与重组白细胞干扰素（ifn - α）进行了比较研究。研究结果不仅证实了IFN- γ对HLA-DR表达调节的选择性活性，而不是IFN- α，而且还表明胶质瘤细胞系及其克隆对两种类型IFN的反应具有明显的异质性。例如，一个可诱导细胞系的所有3个克隆都可以被调节表达HLA-DR，而来自一个不可诱导细胞系的5个克隆中只有2个可以被调节。这种异质性似乎不是由于这些细胞表面缺乏ifn - γ受体，因为几乎所有的细胞系或克隆（19个中的17个）对ifn - γ有反应，通过诱导或增强HLA-DR或HLA-A， -B和-C（或两者）的表达。诱导的异质性也在ifn - γ治疗后不表达HLA-DR的胶质瘤系克隆之间得到证实。其中一个克隆产生的HLA-DR足够多，可以通过免疫沉淀和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析来证实。
The modulation of HLA-DR and HLA-A, -B, and -C by human recombinant immune interferon (IFN-gamma) was studied on 10 malignant glioma cell lines established in our laboratory, on 8 clones or subclones derived from these lines, and on a fetal astrocyte cell line. Comparative studies were performed with recombinant leukocyte interferon (IFN-alpha). The results not only confirmed the selective activity of IFN-gamma on the modulation of HLA-DR expression, as opposed to that of IFN-alpha, but also demonstrated a marked heterogeneity in the response of glioma cell lines and their clones to the two types of IFN tested. For example, all 3 clones of an inducible cell line could be modulated to express HLA-DR, whereas only 2 of 5 clones derived from a noninducible line were modulated. This heterogeneity did not seem to be due to the absence of the receptor for IFN-gamma on the surface of these cells, since almost all of the cell lines or clones tested (17 of 19) responded to IFN-gamma by the induction or enhancement of the expression for either HLA-DR or HLA-A, -B, and -C (or both). The heterogeneity of induction was also demonstrated between clones derived from a glioma line that did not express HLA-DR after IFN-gamma treatment. The production of HLA-DR by one of the clones was abundant enough to be confirmed by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.