Regulation of IGF-I, IGFBP-4 and IGFBP-5 gene expression by loading in mouse skeletal muscle
Regulation of IGF-I, IGFBP-4 and IGFBP-5 gene expression by loading in mouse skeletal muscle
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DOI：
10.1016/s0014-5793(99)01469-6
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发表时间：
1999-11-19
期刊：
影响因子：
3.5
通讯作者：
Lebacq, J
中科院分区：
文献类型：
--
作者：
Awede, B;Thissen, JP;Lebacq, J
研究了超负荷或空载 8 天的小鼠后肢骨骼肌中 IGF-I、IGFBP-4 和 IGFBP-5 的基因表达。超负荷导致比目鱼肌肥大 15%，与 IGF-I 转录物水平增加 60% 和 IGFBP-4 mRNA 水平加倍相关。 IGFBP-5 mRNA 水平降低至对照值的三分之一。 IGFBPs mRNA 的变化总是先于 IGF-I 基因表达的变化。后肢悬浮卸载导致比目鱼肌萎缩（20%），表型向快速型转变，与 IGF-I mRNA 瞬时减少（30%）和 IGFBP-5 转录物持续增加（X2）相关。在空载或超载的比目鱼肌中，IGFBP 表达的这些变化表明它们可能在骨骼肌适应负荷变化中发挥作用。 (C) 1999 年欧洲生化学会联合会。
Gene expression of IGF-I, IGFBP-4 and IGFBP-5 was studied in hindlimb skeletal muscle of mice, which were either overloaded or unloaded for 8 days. Overloading induced a 15% hypertrophy in soleus muscle associated with a 60%, increase of IGF-I transcript levels and a doubling of IGFBP-4 mRNA levels. IGFBP-5 mRNA levels were decreased to one third of the control value. Changes in IGFBPs mRNA always preceded changes in IGF-I gene expression. Unloading by hindlimb suspension resulted in atrophy of soleus muscle (20%) and phenotype change towards the fast type associated with a transient decrease of IGF-I mRNA (30%) and a sustained increase (X2) of IGFBP-5 transcript. These alterations in IGFBPs expression, in unloaded or overloaded soleus, suggest that they may play a role in skeletal muscle adaptation to changes in loading. (C) 1999 Federation of European Biochemical Societies.