DNA Concentration Regulator That can be Driven for a Long Time
DNA Concentration Regulator That can be Driven for a Long Time
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DOI：
10.1007/s00354-022-00173-3
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发表时间：
2022-05
影响因子：
2.6
通讯作者：
T. Nakakuki;Keiji Murayama;H. Asanuma
中科院分区：
文献类型：
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作者：
T. Nakakuki;Keiji Murayama;H. Asanuma
分子机器人是一种自主的微纳米机器，它安装了连接传感器和执行器系统的分子控制器。分子机器人的一个主要控制问题是保持执行器的运行在期望的水平上。这就需要一个调节器来调节系统中特定DNA链的浓度到所需的水平，以便在由DNA链之间的分子相互作用组成的化学反应系统中适当地实现。然而，尽管对DNA浓度调控的研究成果较多，但存在一个主要问题：由于栅链和燃料链的有限性问题，调控通常只能在有限的时间内起作用。控制器的工作时间是一项重要的控制性能指标，是其实际应用的前提。在本研究中，受先前报道的用于可再生PID控制器的基于偶氮苯的光反应方法的启发，提出了简化到实验可行水平的可再生DNA浓度调节器的设计。结果表明，通过光照射及时切换“调节模式”和“浓度恢复模式”的方法可以解决DNA浓度调节器长时间运行的问题。
A molecular robot is an autonomous micro/nano machine that mounts a molecular controller connecting the sensor and actuator systems. A major control problem of a molecular robot is to maintain the actuator operation at the desired level. This requires a regulator that adjusts the concentration of a specific DNA strand of a system to the desired level to be appropriately implemented on a chemical reaction system made of molecular interactions among DNA strands. However, although there are several research results on DNA concentration regulators, there is a major problem: the regulators normally work only within a limited period because of the finiteness problem of gate and fuel strands. The operating time of the controller is an important control performance index and is a prerequisite for its practical applications. In this study, the design of a renewable DNA concentration regulator simplified to an experimentally feasible level is proposed, inspired by the previously reported azobenzene-based photo-reaction method used for a renewable PID controller. It is shown that the methodology that timely switches the operation between “regulator mode” and “concentration recovery mode” by light irradiation can serve as a solution for the long-time operation of the DNA concentration regulator.