Biocompatibility and degradation characteristics of PLGA-based electrospun nanofibrous scaffolds with nanoapatite incorporation
Biocompatibility and degradation characteristics of PLGA-based electrospun nanofibrous scaffolds with nanoapatite incorporation
复制标题
DOI：
10.1016/j.biomaterials.2012.06.018
复制
发表时间：
2012-10-01
期刊：
影响因子：
14
通讯作者：
Jansen, John A.
中科院分区：
文献类型：
--
作者：
Ji, Wei;Yang, Fang;Jansen, John A.
本研究旨在探讨纳米磷灰石颗粒（nAp）掺入对聚乳酸-羟基乙酸酯（PLGA）基纳米纤维支架降解特性和生物相容性的影响。制备了尼龙/聚己内酯（PCL）共混（w/w = 3/1）复合聚合物电纺丝支架，nAp掺入量为0 ~ 30 wt% （n0 ~ n30）。首先对所获得的支架进行形态、物理和化学表征，然后进行体外降解研究。此外，将未降解状态和3周预降解状态的n0和n30直接或在不锈钢网箱中皮下植入大鼠，以评估体内组织反应。结果表明，nAp的掺入对降解过程中的ph水平产生了依赖于nAp量的缓冲作用，并且基于分子量分析延迟了聚合物的降解。在生物相容性方面，在4周的皮下植入过程中，nAp的掺入显著改善了组织反应，显示炎症细胞（单核细胞/巨噬细胞）的浸润减少，支架周围的异物巨细胞（FBGCs）的形成减少。植入支架1周后，各组细胞因子表达（基因和蛋白水平）相似，但基因水平上tnf - α和tgf - β以及蛋白水平上GRO-KC差异不大。本研究结果表明，弱碱性盐nAp杂交能有效控制PLGA材料体内组织不良反应，有利于优化不同PLGA生物医学器械的最终临床应用。(C) 2012 Elsevier Ltd.版权所有。
The aim of current study was to evaluate the effect of nano-apatitic particles (nAp) incorporation on the degradation characteristics and biocompatibility of poly(lactide-co-glycolide) (PLGA)-based nanofibrous scaffolds. Composite PLGA/poly(epsilon-caprolactone) (PCL) blended (w/w = 3/1) polymeric electrospun scaffolds with 0-30 wt% of nAp incorporation (n0-n30) were prepared. The obtained scaffolds were firstly evaluated by morphological, physical and chemical characterization, followed by an in vitro degradation study. Further, n0 and n30 in both virgin and 3-week pre-degraded status were subcutaneously implanted in rats, either directly or in stainless steel mesh cages, to evaluate in vivo tissue response. The results showed that the incorporation of nAp yields an nAp amount-dependent buffering effect on pH-levels during degradation and delayed polymer degradation based on molecular weight analysis. Regarding biocompatibility, nAp incorporation significantly improved the tissue response during a 4-week subcutaneous implantation, showing less infiltration of inflammatory cells (monocyte/macrophages) as well as less foreign body giant cells (FBGCs) formation surrounding the scaffolds. Similar cytokine expression (gene and protein level) was observed for all groups of implanted scaffolds, although marginal differences were found for TNF-alpha and TGF-beta at gene level as well as GRO-KC at protein level after 1 week of implantation. The results of the current study indicate that hybridization of the weak alkaline salt nAp is effective to control the in vivo adverse tissue reaction of PLGA materials, which is beneficial for optimizing final clinical application of different PLGA-based biomedical devices. (C) 2012 Elsevier Ltd. All rights reserved.