Recombinant human basic fibroblast growth factor (bFGF) stimulates periodontal regeneration in class II furcation defects created in beagle dogs
Recombinant human basic fibroblast growth factor (bFGF) stimulates periodontal regeneration in class II furcation defects created in beagle dogs
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DOI：
10.1034/j.1600-0765.2003.00640.x
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发表时间：
2003-02-01
影响因子：
3.5
通讯作者：
Okada, H
中科院分区：
文献类型：
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作者：
Murakami, S;Takayama, S;Okada, H
最近研究了几种生长因子（或细胞因子）作为牙周组织再生的潜在治疗剂。本研究的目的是评估牙周组织再生，包括新骨和牙骨质的形成，局部应用重组碱性成纤维细胞生长因子（bFGF，FGF-2）的分叉II类缺损。在6只比格犬中通过手术产生12个分叉II类骨缺损，然后将重组bFGF（30 μ g/部位）+凝胶状载体局部应用于骨缺损。应用后6周，分析牙周再生。在所有的网站，其中应用碱性成纤维细胞生长因子，牙周膜形成与新的牙骨质沉积和新骨形成的组织形态计量学观察，在量大于在控制网站。碱性FGF应用部位表现出显著的再生，表现为新骨形成率（NBR）（83.6 +/- 14.3%）、新骨小梁形成率（NTBR）（44.1 +/- 9.5%）和新牙骨质形成率（NCR）（97.0 +/- 7.5%）。相比之下，在仅载体位点中，NBR、NTBR和NCR分别为35.4 +/-8.9%、16.6 +/-6.2%和37.2 +/-15.1%。此外，在检查的bFGF应用部位中未观察到上皮向下生长、关节强直或牙根吸收的情况。目前的研究结果表明，局部应用bFGF可以提高相当大的牙周再生在人工创建的分叉II类骨缺损的比格犬。
Several growth factors (or cytokines) have been recently investigated for their use as potential therapeutics for periodontal tissue regeneration. The objective of this study was to evaluate periodontal tissue regeneration, including new bone and cementum formation, following topical application of recombinant basic fibroblast growth factor (bFGF, FGF-2) to furcation class II defects. Twelve furcation class II bone defects were surgically created in six beagle dogs, then recombinant bFGF (30 mug/site) + gelatinous carrier was topically applied to the bony defects. Six weeks after application, periodontal regeneration was analyzed. In all sites where bFGF was applied, periodontal ligament formation with new cementum deposits and new bone formation was observed histomorphometrically, in amounts greater than in the control sites. Basic FGF-applied sites exhibited significant regeneration as represented by the new bone formation rate (NBR) (83.6 +/- 14.3%), new trabecular bone formation rate (NTBR) (44.1 +/- 9.5%), and new cementum formation rate (NCR) (97.0 +/- 7.5%). In contrast, in the carrier-only sites, the NBR, NTBR, and NCR were 35.4 +/- 8.9%, 16.6 +/- 6.2%, and 37.2 +/- 15.1%, respectively. Moreover, no instances of epithelial down growth, ankylosis, or root resorption were observed in the bFGF-applied sites examined. The present results indicate that topical application of bFGF can enhance considerable periodontal regeneration in artificially created furcation class II bone defects of beagle dogs.