Endocytosis and the development of cell polarity in yeast require a dynamic F-actin cytoskeleton
Endocytosis and the development of cell polarity in yeast require a dynamic F-actin cytoskeleton
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DOI：
10.1016/s0960-9822(00)00859-9
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发表时间：
2000-12-14
期刊：
影响因子：
9.2
通讯作者：
Ayscough, KR
中科院分区：
文献类型：
--
作者：
Ayscough, KR
使用能使丝状肌动蛋白（F - 肌动蛋白）解聚的药物进行的研究表明，完整的肌动蛋白细胞骨架对于酵母细胞的极性分泌非常重要[1,2]。然而，为了探究此类过程所需的动态周转水平，需要使用能赋予F - 肌动蛋白稳定特性的药物或突变体。鬼笔环肽是唯一一种在体内和体外都能稳定F - 肌动蛋白结构且容易获取的药物[3 - 6]。已经培育出了缺失两个ABC多药耐药转运蛋白基因的酵母菌株，这使得通常对鬼笔环肽有抗性的酵母细胞对其作用变得敏感。用鬼笔环肽处理这些细胞会对肌动蛋白细胞骨架产生迅速而显著的影响，导致细胞内单个大型肌动蛋白结构的积累。然而，这些结构仍然包含通常与皮质肌动蛋白斑相关的成分。研究发现动态的肌动蛋白细胞骨架对于细胞极性的产生和胞吞作用至关重要。
Studies using drugs that cause the disassembly of filamentous actin (F-actin) have demonstrated the importance of an intact actin cytoskeleton for polarised secretion by yeast cells [1,2], To address the level of dynamic turnover needed for such processes, however, drugs or mutants that confer stabilising properties on F-actin are needed. Jasplakinolide is the only readily available drug that stabilises F-actin structures both in vivo and in vitro [3-6]. Yeast strains have been generated in which two of the ABC multidrug resistance transporter genes have been deleted, rendering normally jasplakinolide-resistant yeast cells sensitive to its effects. Treatment of these cells with jasplakinolide caused rapid and dramatic effects on the actin cytoskeleton, resulting in the accumulation of single large actin structures in cells. These structures, however, still contained components that are normally associated with cortical actin patches. A dynamic actin cytoskeleton was found to be critical for the generation of cell polarity and endocytosis.