Mechanism and regulation of cytoplasmic dynein.
Mechanism and regulation of cytoplasmic dynein.
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DOI：
10.1146/annurev-cellbio-100814-125438
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发表时间：
2015
影响因子：
11.3
通讯作者：
Reck-Peterson SL
中科院分区：
文献类型：
--
作者：
Cianfrocco MA;DeSantis ME;Leschziner AE;Reck-Peterson SL
直到最近，动力蛋白是最不了解的细胞骨架马达。然而，大量新的结构、机制和细胞生物学数据正在揭示这种复杂的负端导向的微管马达是如何工作的。细胞质动力蛋白-1在大多数真核生物中执行广泛的功能，既在间期，其中它运输细胞器，蛋白质，mRNA和病毒，也在有丝分裂和减数分裂中。动力蛋白或其调节因子的突变与神经发育和神经退行性疾病有关。在这里，我们开始通过提供一个综合的最近的数据来描述动力蛋白的机械化学循环的当前模型。接下来，我们讨论动力蛋白的调节剂，特别关注那些直接与电机相互作用，以调节其招聘微管，启动货物运输，或激活负端定向运动。
Until recently, dynein was the least understood of the cytoskeletal motors. However, a wealth of new structural, mechanistic, and cell biological data is shedding light on how this complicated minus-end–directed, microtubule-based motor works. Cytoplasmic dynein-1 performs a wide array of functions in most eukaryotes, both in interphase, in which it transports organelles, proteins, mRNAs, and viruses, and in mitosis and meiosis. Mutations in dynein or its regulators are linked to neurodevelopmental and neurodegenerative diseases. Here, we begin by providing a synthesis of recent data to describe the current model of dynein’s mechanochemical cycle. Next, we discuss regulators of dynein, with particular focus on those that directly interact with the motor to modulate its recruitment to microtubules, initiate cargo transport, or activate minus-end–directed motility.