Primary transcripts of microRNAs encode regulatory peptidesPrimary transcripts of microRNAs encode regulatory peptides
Primary transcripts of microRNAs encode regulatory peptidesPrimary transcripts of microRNAs encode regulatory peptides
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DOI：
10.1038/nature14346
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发表时间：
2015-04-02
期刊：
影响因子：
64.8
通讯作者：
Combier, Jean-Philippe
中科院分区：
文献类型：
--
作者：
Lauressergues, Dominique;Couzigou, Jean-Malo;Combier, Jean-Philippe
MicroRNA（miRNAs）是小的调节RNA分子，其通过结合并切割其信使RNA或以其他方式抑制其翻译成蛋白质来抑制特定靶基因的表达（1）。miRNA被转录为更大的初级转录物（pri-miRNA），其功能尚未完全了解。在这里，我们表明，植物pri-miRNAs包含短的开放阅读框架序列，编码调节肽。蒺藜苜蓿的pri-miR 171 b和拟南芥的pri-miR 165 a产生肽，我们分别称之为miPEP 171 b和miPEP 165 a，其增强其相应成熟miRNAs的积累，导致参与根发育的靶基因的下调。miRNA编码肽（miPEP）作用的机制涉及增加pri-miRNA的转录。A. thaliana和M. truncatula编码活性miPEP，表明miPEP广泛分布于整个植物界。应用于植物的合成miPEP 171 b和miPEP 165 a肽特异性地触发miR 171 b和miR 165 a的积累，分别导致侧根发育的减少和主根生长的刺激，这表明miPEP可能具有农学应用。
MicroRNAs (miRNAs) are small regulatory RNA molecules that inhibit the expression of specific target genes by binding to and cleaving their messenger RNAs or otherwise inhibiting their translation into proteins(1). miRNAs are transcribed as much larger primary transcripts (pri-miRNAs), the function of which is not fully understood. Here we show that plant pri-miRNAs contain short open reading frame sequences that encode regulatory peptides. The pri-miR171b of Medicago truncatula and the pri-miR165a of Arabid-opsis thaliana produce peptides, which we term miPEP171b and miPEP165a, respectively, that enhance the accumulation of their corresponding mature miRNAs, resulting in downregulation of target genes involved in root development. The mechanism of miRNA-encoded peptide (miPEP) action involves increasing transcription of the pri-miRNA. Five other pri-miRNAs of A. thaliana and M. truncatula encode active miPEPs, suggesting that miPEPs are widespread throughout the plant kingdom. Synthetic miPEP171b and miPEP165a peptides applied to plants specifically trigger the accumulation of miR171b and miR165a, leading to reduction of lateral root development and stimulation of mainroot growth, respectively, suggesting that miPEPs might have agronomical applications.