Photoacoustic tomography can detect cerebral hemodynamic alterations in a neonatal rodent model of hypoxia-ischemia.
Photoacoustic tomography can detect cerebral hemodynamic alterations in a neonatal rodent model of hypoxia-ischemia.
复制标题
DOI：
10.55782/ane-2012-1898
复制
发表时间：
2012-09
影响因子：
1.4
通讯作者：
C. Sussman;Candace Rossignol;Qizhi Zhang;Huabei Jiang;T. Zheng;D. Steindler;L. Young;M. Weiss
中科院分区：
文献类型：
--
作者：
C. Sussman;Candace Rossignol;Qizhi Zhang;Huabei Jiang;T. Zheng;D. Steindler;L. Young;M. Weiss
缺氧缺血性脑病（Hypoxic-Ischemic Encephalopathy，HIE）是儿童神经功能缺损的最常见原因之一。脑血流量（CBF）减少，如HIE所见，导致神经元损伤尚未进行实时评估。光声断层扫描（PAT）是一种光学成像形式，可以通过血红蛋白光吸收的变化以无创、非电离的方式检测脑血流动力学变化。此外，这项技术有可能实时捕获脑血容量（CBV）波动，也许还有CBF变化。我们假设PAT可以检测到缺氧缺血新生儿模型中脑血红蛋白光吸收的减少，从而检测到CBF的减少。为了研究，P7大鼠接受右颈动脉结扎，暴露于8%的氧气中60分钟，同时每20分钟用PAT成像。通过平均光吸收（MOA）测量的脑血流动力学变化计算为较基线的变化。使用线性混合模型分析了全球和区域MOA。缺氧时，右半球的总体MOA比左半球减少。在左侧和右侧的中间和后部皮质区域之间检测到MOA的区域差异。使用免疫组织化学确认损伤。我们的结论是减少全球和区域MOA，因此CBF，可以确定PAT在新生大鼠模型的HIE。这是文献中描述的第一项利用新生大鼠HIE模型以非侵入性和近实时方式证明脑血流动力学体内变化的研究。
Hypoxic-Ischemic Encephalopathy (HIE) is one of the most recognized causes of neurological deficits in children. Cerebral blood flow (CBF) reductions, as seen with HIE, resulting in neuronal injury have not been evaluated in real-time. Photoacoustic Tomography (PAT) is a form of optical imaging which can detect cerebral hemodynamic alterations in a noninvasive, non-ionizing fashion via changes in hemoglobin optical absorption. Further, this technology has the potential to capture cerebral blood volume (CBV) fluctuations and perhaps CBF changes in real-time. We hypothesized that PAT can detect a reduction in cerebral hemoglobin optical absorption, and therefore CBF, in a neonatal model of hypoxia-ischemia. To investigate, P7 rats underwent right carotid artery ligation and exposure to 8 percent oxygen for 60 minutes while imaged with PAT every 20 minutes. Cerebral hemodynamic alterations, as measured by mean optical absorption (MOA), were calculated as a change from baseline. Global and regional MOA was analyzed using a linear mixed model. Global MOA was reduced within the right hemisphere as compared to the left during hypoxia. Regional differences in MOA were detected between the left and right sides for the middle and posterior cortical regions. Injury was confirmed using immunohistochemistry. We conclude that a reduction in global and regional MOA, and hence CBF, could be identified by PAT in a neonatal rat model of HIE. This is the first study described in the literature utilizing a neonatal rat model of HIE to demonstrate in vivo alterations in cerebral hemodynamics in a non-invasive and near real-time fashion.