A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis
A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis
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来自羊草的新型盐诱导基因 LcSAIN2 可增强转基因拟南芥的耐盐性
DOI：
10.1016/j.plaphy.2012.12.014
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发表时间：
2013-03-01
影响因子：
6.5
通讯作者：
Liu, Gongshe
中科院分区：
文献类型：
--
作者：
Li, Xiaoxia;Gao, Qiong;Liu, Gongshe
盐胁迫影响植物的生长发育，限制作物的产量。羊草在各种环境和土壤条件下都能生长良好，是小麦族中一种良好的野生资源。利用454高通量测序技术，从绵羊草中筛选出大量盐胁迫响应基因。本研究克隆了一个新的盐诱导基因及其启动子，并将其命名为LcSAIN 2（Leymus chinensis salt-induced 2）。生物信息学分析预测LcSAIN 2具有一个跨膜螺旋，定位于细胞核。在烟草叶片细胞中的亚细胞定位实验也表明它主要定位于细胞核。在LcSAIN 2基因的启动子区发现了几个胁迫响应元件。表达分析证实LcSAIN 2受盐、PEG、阿坝和冷胁迫的诱导，尤其是高盐胁迫。在拟南芥中过量表达LcSAIN 2可以通过积累可溶性糖和游离脯氨酸等渗透调节物质以及提高一些逆境应答转录因子和关键基因的表达水平来增强转基因植物的耐盐性。这些结果表明，LcSAIN 2基因可能在小麦耐盐胁迫中起重要的正向调节作用，可作为提高小麦等作物耐盐性的候选基因。(C)2013年Elsevier Masson SAS。All rights reserved.
Salt stress affects plant growth and development, and limits the productivity of crops. Sheepgrass can grow well under various environmental and soil conditions and is a good wild resource in Triticeae. Using 454 high throughout sequencing technique, a large number of salt stress responsive genes have been picked out from sheepgrass. In this study, a novel salt-induced gene and its promoter were cloned and the gene was designated as LcSAIN2 (Leymus chinensis salt-induced 2). Bioinformatics analysis predicted that LcSAIN2 has one transmembrane helix and is localized in nucleus. Experiments of subcellular localization in tobacco leaf cells also indicated that it was mainly localized in nucleus. Several stress responsive elements were found in the promoter region of the LcSAIN2 gene. The expression analysis confirmed that LcSAIN2 was induced by salinity, PEG, ABA, and cold stresses, especially by high salinity. Overexpression of LcSAIN2 in Arabidopsis enhanced salt tolerance of transgenic plants by accumulating osmolytes, such as soluble sugars and free proline, and improving the expression levels of some stress-responsive transcription factors and key genes. Our results suggest that LcSAIN2 might play an important positive modulation role in salt stress tolerance and be a candidate gene utilized for enhancing stress tolerance in wheat and other crops. (C) 2013 Elsevier Masson SAS. All rights reserved.