Histological study on ouabain immunoreactivities in the mammalian hypothalamus
Histological study on ouabain immunoreactivities in the mammalian hypothalamus
复制标题
哺乳动物下丘脑哇巴因免疫反应性的组织学研究
DOI：
10.1016/0304-3940(92)90881-7
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发表时间：
1992
影响因子：
2.5
通讯作者：
J. Ochi
中科院分区：
文献类型：
--
作者：
H. Yamada;M. Naruse;K. Naruse;H. Demura;Hakuo Takahashi;M. Yoshimura;J. Ochi
内源性洋地黄样因子(EDLF)是最近从人血浆中分离得到的一种植物来源的内源性洋地黄样物质(EDLS)，被认为与假想的体液因子类似。为了验证下丘脑产生EDLS的假设，我们制备了一种哇宾特异性抗体，并将其应用于大鼠和猕猴的大脑。在两种动物的下丘脑中均观察到哇巴因免疫反应。免疫阳性神经元分布于下丘脑室旁核、视上核及其他区域。在下丘脑-神经垂体区可见丰富的神经纤维。这些结果有力地支持了哺乳动物脑中存在包括EDLF在内的顺式-反式顺式类固醇的可能性。
The endogenous degitalis-like factor (EDLF) which has recently been purified from human plasma and identified as ‘ouabain’, acis-trans-cissteroid of plant origin, is thought to be similar to the hypothetical humoral factor, ‘endogenous digitalis-like substance (EDLS)’. In order to examine the hypothesis that EDLS is produced in the hypothalamus, we prepared an ouabian-specific antibody, and applied it to rat and macaque brains. Ouabain immunoreactivities were observed in the hypothalamus of both species. The immunopositive neurons were distributed in paraventricular and supraoptic nuclei, and some other hypothalamic regions. Their nerve fibers were seen abundantly in the hypothalamo-neurohypophysial regions. These results strongly support the possibility of existence ofcis-trans-cissteroid including EDLF in mammalian brain.
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kelly,RA;O'Hara,DS;Canessa,ML;Mitch,WE;Smith,TW
通讯作者：
Smith,TW