BRANCHING MUTANT RMS-2 IN PISUM-SATIVUM - GRAFTING STUDIES AND ENDOGENOUS INDOLE-3-ACETIC-ACID LEVELS
BRANCHING MUTANT RMS-2 IN PISUM-SATIVUM - GRAFTING STUDIES AND ENDOGENOUS INDOLE-3-ACETIC-ACID LEVELS
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DOI：
10.1104/pp.104.3.953
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发表时间：
1994-03-01
期刊：
影响因子：
7.4
通讯作者：
MURFET, IC
中科院分区：
文献类型：
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作者：
BEVERIDGE, CA;ROSS, JJ;MURFET, IC
豌豆（Pisum sativum L.）的Rms-2基因，它保持顶端优势，和内源性游离吲哚-3-乙酸（IAA）水平的影响，用于确定的生理位点的行动。在突变体RMS-2接穗，通常产生侧枝低于节点3和高于节点7，顶端优势几乎完全恢复嫁接到RMS-2（野生型）股票。在相互移植，rms-2堆栈没有促进分支野生型芽。总之，这些结果表明，Rms-2基因通过控制在根和/或子叶和芽中产生的可转位（类豌豆）物质的合成来抑制豌豆芽中的分支。在所有阶段，包括阶段在空中侧芽开始生长，IAA的水平在rms-a芽升高（高达5倍）相比，在野生型芽。rms-2植物的节间长度比野生型植物少40%，与野生型植物相比，突变体植物分配到地上部的干重显著高于分配到根的干重。手工制作的野生型股票没有正常化的IAA水平或节间长度在rms-2接穗，即使它抑制分支，这表明参与的RMS-2的IAA水平和节间长度的控制可能仅限于在拍摄过程。
Isogenic lines of pea (Pisum sativum L.) were used to determine the physiological site of action of the Rms-2 gene, which maintains apical dominance, and its effect on endogenous free indole-3-acetic acid (IAA) levels. In mutant rms-2 scions, which normally produce lateral branches below node 3 and above node 7, apical dominance was almost fully restored by grafting to Rms-2 (wildtype) stocks. In the reciprocal grafts, rms-2 stacks did not promote branching in wild-type shoots. Together, these results suggest that the Rms-2 gene inhibits branching in the shoot of pea by controlling the synthesis of a translocatable (hormone-like) substance that is produced in the roots and/or cotyledons and in the shoot. At all stages, including the stage at which aerial lateral buds commence outgrowth, the level of IAA in rms-a shoots was elevated (up to 5-fold) in comparison with that in wild-type shoots. The internode length of rms-2 plants was 40% less than in wild-type plants, and the mutant plants allocated significantly more dry weight to the shoot than to the root in comparison with wild-type plants. Crafting to wild-type stocks did not normalize IAA levels or internode length in rms-2 scions, even though it inhibited branching, suggesting that the involvement of Rms-2 in the control of IAA level and internode length may be confined to processes in the shoot.