Designed Intercalators for Modification of DNA Origami Surface Properties
Designed Intercalators for Modification of DNA Origami Surface Properties
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DOI：
10.1002/chem.201500086
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发表时间：
2015-06-22
影响因子：
4.3
通讯作者：
Niemeyer, Christof M.
中科院分区：
文献类型：
--
作者：
Brglez, Josipa;Nikolov, Pavel;Niemeyer, Christof M.
据报道，基于用含有酯化脂肪酸或低聚乙二醇残基的侧链衍生的吖啶，通过与设计的嵌入剂的非共价相互作用来修饰DNA折纸纳米结构的主链特性。光谱分析表明这些嵌入剂与 DNA 折纸结构结合。原子力显微镜研究表明，嵌入剂结合不会影响结构完整性，但会导致带高负电荷的纳米结构的表面性质发生改变，正如它们与固体云母或石墨载体的相互作用所证明的那样。此外，嵌入剂和折纸结构之间的非共价相互作用导致细胞摄取的改变，如使用两种不同真核细胞系的共聚焦显微镜研究所示。因此，嵌入剂方法为定制 DNA 纳米结构的表面特性提供了一种潜在的方法。
The modification of the backbone properties of DNA origami nanostructures through noncovalent interactions with designed intercalators, based on acridine derivatized with side chains containing esterified fatty acids or oligo(ethylene glycol) residues is reported. Spectroscopic analyses indicate that these intercalators bind to DNA origami structures. Atomic force microscopy studies reveal that intercalator binding does not affect the structural intactness but leads to altered surface properties of the highly negatively charged nanostructures, as demonstrated by their interaction with solid mica or graphite supports. Moreover, the noncovalent interaction between the intercalators and the origami structures leads to alteration in cellular uptake, as shown by confocal microscopy studies using two different eukaryotic cell lines. Hence, the intercalator approach offers a potential means for tailoring the surface properties of DNA nanostructures.